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Transgenic Cotton

Methods and Protocols

  • Baohong Zhang

Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

  3. Transformation

  4. Genome Editing and Vigs

    1. Front Matter
      Pages 93-93
    2. Chao Li, Baohong Zhang
      Pages 95-104
    3. Caiping Cai, Xinyu Wang, Baohong Zhang, Wangzhen Guo
      Pages 105-119
  5. Detection

  6. Application

  7. Risk Assessment

    1. Front Matter
      Pages 307-307
  8. Back Matter
    Pages 323-327

About this book

Introduction

This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic cotton, principle and methods for making transgenic cotton, creating gene knockout lines, foreign gene copy and expression in transgenic plants, improvements to cotton using transgenic technology, and monitoring the potential impact of transgenic cotton on environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and cutting-edge, Transgenic Cotton: Methods and Protocols 2nd aims to be a resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology, and agricultural sciences.

Keywords

Agrobacterium pollen tube pathway ELISA CRISPR/Cas 9 miRNA

Editors and affiliations

  • Baohong Zhang
    • 1
  1. 1.Department of BiologyEast Carolina UniversityGreenvilleUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8952-2
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8951-5
  • Online ISBN 978-1-4939-8952-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site