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Barley

Methods and Protocols

  • Wendy A. Harwood

Part of the Methods in Molecular Biology book series (MIMB, volume 1900)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ludmila Ohnoutkova
    Pages 7-19
  3. William T. B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, Jerome D. Franckowiak
    Pages 21-36
  4. Ludmila Ohnoutkova, Tomas Vlcko, Mentewab Ayalew
    Pages 37-52
  5. Patricio Esteves, François J. Belzile
    Pages 53-71
  6. Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, Nils Stein
    Pages 73-94
  7. Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, Fiona M. Doohan
    Pages 95-114
  8. Alison Hinchliffe, Wendy A. Harwood
    Pages 115-126
  9. Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Michał Słota, Dorota Swiergolik, Iwona Szarejko
    Pages 127-151
  10. Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, Isabelle Colas
    Pages 167-179
  11. Jenny A. Morris, Pete E. Hedley
    Pages 181-194
  12. Goetz Hensel, Jochen Kumlehn
    Pages 195-215
  13. Tom Lawrenson, Wendy A. Harwood
    Pages 217-232
  14. Amina Abed, Gaétan Légaré, Sonia Pomerleau, Jérôme St-Cyr, Brian Boyle, François J. Belzile
    Pages 233-252
  15. Karolina Chwialkowska, Urszula Korotko, Miroslaw Kwasniewski
    Pages 253-268
  16. Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P. G. Calixto, Linda Milne et al.
    Pages 269-281
  17. Micha Bayer, Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell et al.
    Pages 283-310
  18. Back Matter
    Pages 311-313

About this book

Introduction

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

Keywords

Plant sciences Cereal genomics Crop genetics Mutation breeding Tissue culture Genotyping Gene expression

Editors and affiliations

  • Wendy A. Harwood
    • 1
  1. 1.Crop Transformation Group, Department of Crop GeneticsJohn Innes CentreNorwichUK

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8944-7
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8942-3
  • Online ISBN 978-1-4939-8944-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site