Atomic Force Microscopy

Methods and Protocols

  • Nuno C. Santos
  • Filomena A. Carvalho

Part of the Methods in Molecular Biology book series (MIMB, volume 1886)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Imaging

    1. Front Matter
      Pages 1-1
    2. Peter Eaton, Krystallenia Batziou
      Pages 3-28
    3. Simon D. Connell, George R. Heath, James A. Goodchild
      Pages 29-44
    4. Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, Cédric Godefroy et al.
      Pages 45-59
    5. Jiang Pi, Jiye Cai
      Pages 99-113
  3. Single-Molecule Force Spectroscopy

    1. Front Matter
      Pages 115-115
    2. Jianli Liu, Wenhui Li, Xuejie Zhang, Yan Feng, Xiaohong Fang
      Pages 153-162
    3. Joseph D. Unsay, Ana J. García-Sáez
      Pages 191-202
    4. Ricardo H. Pires, Mihaela Delcea, Stephan B. Felix
      Pages 203-217
  4. Studies of Bacteria and Virus in AFM

    1. Front Matter
      Pages 219-219
    2. Yoo Jin Oh, Peter Hinterdorfer
      Pages 221-231
    3. Marco M. Domingues, Mário R. Felício, Sónia Gonçalves
      Pages 233-242
    4. Yukun Guo, Wouter H. Roos
      Pages 243-257
    5. Álvaro Ortega-Esteban, Natália Martín-González, Francisco Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Cármen San MartÚn et al.
      Pages 259-278
  5. AFM Elasticity Studies

    1. Front Matter
      Pages 279-279
    2. Hermann Schillers
      Pages 291-313
    3. Małgorzata Lekka, Joanna Pabijan
      Pages 315-324
  6. AFM Applications in Nanomedicine

    1. Front Matter
      Pages 325-325
    2. Carla P. Gomes, Hugo Oliveira, Andreas Ebner, Peter Hinterdorfer, Ana P. Pêgo
      Pages 327-341
    3. Jan Pribyl, Martin Pešl, Guido Caluori, Ivana Acimovic, Sarka Jelinkova, Petr Dvorak et al.
      Pages 343-353
  7. Back Matter
    Pages 369-372

About this book


This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.


Single-molecule force spectroscopy AFM elasticity Nanomedicine Quantitative imaging Nanoscale structure

Editors and affiliations

  • Nuno C. Santos
    • 1
  • Filomena A. Carvalho
    • 2
  1. 1.Instituto de Medicina Molecular, Faculdade de MedicinaUniversidade de LisboaLisbonPortugal
  2. 2.Instituto de Medicina Molecular, Faculdade de MedicinaUniversidade de LisboaLisbonPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8893-8
  • Online ISBN 978-1-4939-8894-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site