Systems Chemical Biology

Methods and Protocols

  • Slava Ziegler
  • Herbert Waldmann

Part of the Methods in Molecular Biology book series (MIMB, volume 1888)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Suzana Vidic, Marta F. Estrada, Kjersti Gjerde, Vítor E. Santo, Annika Osswald, Michaël Barbier et al.
    Pages 1-20
  3. Masin Abo-Rady, Jessica Bellmann, Michael Glatza, Lara Marrone, Lydia Reinhardt, Santiago Tena et al.
    Pages 21-43
  4. Matthew B. Robers, James D. Vasta, Cesear R. Corona, Rachel Friedman Ohana, Robin Hurst, Manisha A. Jhala et al.
    Pages 45-71 Open Access
  5. Elena S. Reckzeh, Andreas Brockmeyer, Malte Metz, Herbert Waldmann, Petra Janning
    Pages 73-98
  6. Jun Ando, Kosuke Dodo, Katsumasa Fujita, Mikiko Sodeoka
    Pages 99-114
  7. Daniel Bucio-Noble, Crystal Semaan, Mark P. Molloy
    Pages 141-152
  8. Dominic Hoepfner, Gregory McAllister, Gregory R. Hoffman
    Pages 153-174
  9. Mahendra Awale, Jean-Louis Reymond
    Pages 255-272
  10. Ryan Byrne, Gisbert Schneider
    Pages 273-309
  11. Back Matter
    Pages 311-315

About this book


This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level.


Small molecules Cellular networks Computational strategies Cheminformatic approaches Drug repurposing Databases

Editors and affiliations

  • Slava Ziegler
    • 1
  • Herbert Waldmann
    • 2
  1. 1.Department of Chemical BiologyMax Planck Institute of Molecular PhysiologyDortmundGermany
  2. 2.Department of Chemical BiologyMax Planck Institute of Molecular PhysiologyDortmundGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8890-7
  • Online ISBN 978-1-4939-8891-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site