Methods and Protocols

  • Rita Musetti
  • Laura Pagliari

Part of the Methods in Molecular Biology book series (MIMB, volume 1875)

Table of contents

  1. Front Matter
    Pages i-xi
  2. L. Pagliari, R. Musetti
    Pages 1-6
  3. Preparing Plant Material

    1. Front Matter
      Pages 7-7
    2. L. Pagliari, J. Chuche, D. Bosco, D. Thiéry
      Pages 21-36
    3. Kerstin Krüger, Nicola Fiore
      Pages 37-52
    4. Paolo Ermacora, Ruggero Osler
      Pages 53-67
  4. Molecular Analyses

    1. Front Matter
      Pages 69-69
    2. Assunta Bertaccini, Samanta Paltrinieri, Nicoletta Contaldo
      Pages 83-95
    3. Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, Peter Abrahamian
      Pages 117-130
    4. Ioanna Malandraki, Christina Varveri, Nikon Vassilakos
      Pages 151-157
    5. Claudio Ratti, Stefano Minguzzi, Massimo Turina
      Pages 159-169
    6. Nataša Mehle, Tanja Dreo
      Pages 171-186
    7. Davide Pacifico, Simona Abbà, Sabrina Palmano
      Pages 239-251
  5. Site-Specific Analyses

    1. Front Matter
      Pages 253-253
    2. Matthias R. Zimmermann, Torsten Knauer, Alexandra C. U. Furch
      Pages 291-299
    3. Luciana Galetto, Marta Vallino, Mahnaz Rashidi, Cristina Marzachì
      Pages 307-317
  6. Plant-Pathogen Interaction

  7. Back Matter
    Pages 359-362

About this book


This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field.


fluorescent microscopy Macrosteles quadrilineatus DNA extraction phytoplasma vector species PCR assay

Editors and affiliations

  • Rita Musetti
    • 1
  • Laura Pagliari
    • 2
  1. 1.Department of Agricultural, Food, Environmental and Animal SciencesUniversity of UdineUdineItaly
  2. 2.Department of Agricultural, Food, Environmental and Animal SciencesUniversity of UdineUdineItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8836-5
  • Online ISBN 978-1-4939-8837-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site