Methods and Protocols

  • Chengyu Liu
  • Yubin Du

Part of the Methods in Molecular Biology book series (MIMB, volume 1874)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Xin-an Pu, Anthony P. Young, H. Michael Kubisch
    Pages 17-41
  3. Michael G. Zeidler, Thomas L. Saunders
    Pages 43-69
  4. Yubin Du, Wen Xie, Fan Zhang, Chengyu Liu
    Pages 99-114
  5. Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, Colin L. Sweeney
    Pages 139-168
  6. Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama
    Pages 211-228
  7. Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, Takayuki Shindo
    Pages 257-271
  8. Dongshan Yang, Jie Xu, Y. Eugene Chen
    Pages 327-345
  9. Rong Li, Jinxin Miao, Zhongde Wang
    Pages 347-360
  10. Zhiqiang Fan, Min Yang, Misha Regouski, Irina A. Polejaeva
    Pages 373-390
  11. Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, Fuliang Du
    Pages 391-401
  12. Ellen J. Collarini, Philip A. Leighton, Marie-Cecile Van de Lavoir
    Pages 403-430
  13. Jyoti Iyer, Nicole DeVaul, Tyler Hansen, Ben Nebenfuehr
    Pages 431-457
  14. Rie Goto, Taiju Saito, Takahiro Matsubara, Etsuro Yamaha
    Pages 475-487
  15. Daji Luo, Ke Feng, Zuoyan Zhu, Wei Hu
    Pages 489-506
  16. Yuki Shibata, Lingyu Bao, Liezhen Fu, Bingyin Shi, Yun-Bo Shi
    Pages 507-524
  17. Back Matter
    Pages 537-540

About this book


This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.


Gene editing Animal model generation CRISPR TALEN Embryo micromanipulations Genome engineering

Editors and affiliations

  • Chengyu Liu
    • 1
  • Yubin Du
    • 2
  1. 1.National Heart, Lung, and Blood InstituteNational Institutes of HealthBethesdaUSA
  2. 2.National Heart, Lung, and Blood InstituteNational Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8830-3
  • Online ISBN 978-1-4939-8831-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site