Protein Misfolding Diseases

Methods and Protocols

  • Cláudio M. Gomes

Part of the Methods in Molecular Biology book series (MIMB, volume 1873)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Protein Biophysics Assays

    1. Front Matter
      Pages 1-1
    2. Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes
      Pages 3-18
    3. Eri Saijo, Bradley R. Groveman, Allison Kraus, Michael Metrick, Christina D. Orrù, Andrew G. Hughson et al.
      Pages 19-37
    4. Ravit Malishev, Sofiya Kolusheva, Raz Jelinek
      Pages 39-51
    5. Donatella Bulone, Pier Luigi San Biagio, Tatiana Quiñones-Ruiz, Manuel Rosario-Alomar, Igor K. Lednev, Frank T. Robb et al.
      Pages 69-92
    6. Alireza Abdolvahabi, Sanaz Rasouli, Corbin M. Croom, Devon L. Plewman
      Pages 93-108
    7. Devkee M. Vadukul, Youssra K. Al-Hilaly, Louise C. Serpell
      Pages 109-122
    8. Luis M. Blancas-Mejia, Pinaki Misra, Christopher J. Dick, Marta Marin-Argany, Keely R. Redhage, Shawna A. Cooper et al.
      Pages 123-153
  3. Cellular and Proteostasis Assays

    1. Front Matter
      Pages 155-155
    2. Evelien Eenjes, Young Joo Yang-Klingler, Ai Yamamoto
      Pages 157-169
    3. Edward Pokrishevsky, Jeremy Nan, Neil R. Cashman
      Pages 213-224
    4. Steffen J. Sahl, Willianne I. M. Vonk
      Pages 241-251
  4. Protein Folding Recovery and Correction Assays

    1. Front Matter
      Pages 253-253
    2. Giulia Diniz da Silva Ferretti, Danielly C. Ferraz da Costa, Jerson L. Silva, Luciana Pereira Rangel
      Pages 265-277
    3. Pierce T. O’Neil, Alexandra J. Machen, Jackie A. Thompson, Wei Wang, Quyen Q. Hoang, Michael R. Baldwin et al.
      Pages 293-304
    4. Tuane Cristine R. G. Vieira, Jerson L. Silva
      Pages 305-316
    5. Rob van der Kant, Joost van Durme, Frederic Rousseau, Joost Schymkowitz
      Pages 317-333
  5. Back Matter
    Pages 335-338

About this book


This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections on cellular and proteostasis assays as well as assays for protein folding correction and recovery, combining methods such as thermal shift assays, in silico improvement of protein solubility, and compound screening, an important area of research as it may open avenues for therapeutic strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Protein Misfolding Diseases: Methods and Protocols serves as an ideal guide for researchers seeking to advance our knowledge of protein conformational disorders.


Protein biophysics Chaperonopathies Proteostasis Protein folding diseases Conformational disorders Amyloid forming diseases Protein folding correction

Editors and affiliations

  • Cláudio M. Gomes
    • 1
  1. 1.BioISI – Biosystems and Integrative Sciences InstituteFaculty of Sciences University of LisbonLisbonPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8819-8
  • Online ISBN 978-1-4939-8820-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site