Methods and Protocols

  • Narendra Wajapeyee
  • Romi Gupta

Part of the Methods in Molecular Biology book series (MIMB, volume 1870)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Lukas Trixl, Dietmar Rieder, Thomas Amort, Alexandra Lusser
    Pages 1-21
  3. Mingming Chen, Shiqi Mao, Xiaotian Wu, Zhao Ma, Yantao Yang, Christopher J. Krueger et al.
    Pages 23-39
  4. Urszula Waśko, Zeming Zheng, Sanchita Bhatnagar
    Pages 41-50
  5. Lee E. Vandivier, Zachary D. Anderson, Brian D. Gregory
    Pages 51-67
  6. Greggory M. Rice, Razvan Nutiu, Christian M. Gampe
    Pages 81-87
  7. Lina Worpenberg, Tobias Jakobi, Christoph Dieterich, Jean-Yves Roignant
    Pages 89-106
  8. Irene Masiello, Marco Biggiogera
    Pages 165-177
  9. Xiaowei Chen, Yajing Hao, Ya Cui, Zhen Fan, Runsheng Chen
    Pages 189-198
  10. Sandro Andreotti, Stefan Canzar
    Pages 199-208
  11. Gianluigi Lichinchi, Tariq M. Rana
    Pages 209-218
  12. Hironori Adachi, Meemanage D. DeZoysa, Yi-Tao Yu
    Pages 219-235
  13. Yu-Sheng Chen, Hai-Li Ma, Ying Yang, Wei-Yi Lai, Bao-Fa Sun, Yun-Gui Yang
    Pages 237-248
  14. Radoslav Janostiak, Narendra Wajapeyee
    Pages 249-262
  15. Arvindhan Nagarajan, Radoslav Janostiak, Narendra Wajapeyee
    Pages 263-271
  16. Adeline Galvanin, Lilia Ayadi, Mark Helm, Yuri Motorin, Virginie Marchand
    Pages 273-295
  17. Back Matter
    Pages 297-298

About this book


This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more about this field.


Eukaryotic cells yeast meiosis single-cell RNA-sequencing ESAT dsRNAome immune-northern blotting post-transcriptional RNA modification

Editors and affiliations

  • Narendra Wajapeyee
    • 1
  • Romi Gupta
    • 2
  1. 1.Department of PathologyYale University School of MedicineNew HavenUSA
  2. 2.Yale Cancer CenterYale University School of MedicineNew HavenUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8807-5
  • Online ISBN 978-1-4939-8808-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site