Transgenic Plants

Methods and Protocols

  • Sandeep Kumar
  • Pierluigi Barone
  • Michelle Smith

Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Plant Transformation and Vector Construction

    1. Front Matter
      Pages 1-1
    2. Qiudeng Que, Mary-Dell M. Chilton, Sivamani Elumalai, Heng Zhong, Shujie Dong, Liang Shi
      Pages 3-18
    3. David G. J. Mann, Scott A. Bevan, Anthony J. Harvey, Rachelle A. Leffert-Sorenson
      Pages 19-35
    4. Dheeraj Singh Rathore, Evelyn Zuniga-Soto, Ewen Mullins
      Pages 37-48
    5. Polyana Kelly Martins, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayshi, Hugo Bruno Correa Molinari
      Pages 49-65
    6. Andika Gunadi, Eric A. Dean, John J. Finer
      Pages 67-79
    7. Todd Jones, Keith Lowe, George Hoerster, Ajith Anand, Emily Wu, Ning Wang et al.
      Pages 81-93
    8. Bin Tian, Mónica Navia-Urrutia, Yueying Chen, Jordan Brungardt, Harold N. Trick
      Pages 117-130
    9. Sareena Sahab, Matthew J. Hayden, John Mason, German Spangenberg
      Pages 131-152
    10. Tobias Bruegmann, Olaf Polak, Khira Deecke, Julia Nietsch, Matthias Fladung
      Pages 165-177
    11. S. A. Dhekney, S. K. Sessions, M. Brungart-Rosenberg, C. Claflin, Z. T. Li, D. J. Gray
      Pages 191-201
    12. Myron A. Bruce, Jessica L. Shoup Rupp
      Pages 203-223
    13. Joyce Van Eck, Patricia Keen, Michelle Tjahjadi
      Pages 225-234
  3. Genome Editing

    1. Front Matter
      Pages 235-235
    2. Carla Schmidt, Michael Pacher, Holger Puchta
      Pages 237-266
    3. Si Nian Char, Riqing Li, Bing Yang
      Pages 279-293
  4. Utility of Transgenic Traits

    1. Front Matter
      Pages 311-311
    2. Tejinder Mall, Manju Gupta, Tarlochan Singh Dhadialla, Sarria Rodrigo
      Pages 313-342
    3. Laura L. Wayne, Daniel J. Gachotte, Terence A. Walsh
      Pages 367-394
  5. Transgenic Event Characterization

    1. Front Matter
      Pages 395-395
    2. Wei Chen, PoHao Wang
      Pages 397-410
    3. Satyalinga Srinivas Gampala, Bryant Wulfkuhle, Kimberly A. Richey
      Pages 411-417
  6. Back Matter
    Pages 441-444

About this book


This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security.


Transgenic crops Agrobacterium Genome engineering technology Biotechnological applications Plant transformation Genome editing Crop productivity

Editors and affiliations

  • Sandeep Kumar
    • 1
  • Pierluigi Barone
    • 2
  • Michelle Smith
    • 3
  1. 1.Corteva Agriscience™Agriculture Division of DowDuPont™JohnstonUSA
  2. 2.Corteva Agriscience™Agriculture Division of DowDuPont™JohnstonUSA
  3. 3.Corteva Agriscience™Agriculture Division of DowDuPont™JohnstonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8777-1
  • Online ISBN 978-1-4939-8778-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site