Morphogen Gradients

Methods and Protocols

  • Julien Dubrulle

Part of the Methods in Molecular Biology book series (MIMB, volume 1863)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Visualization of Morphogen Gradients

    1. Front Matter
      Pages 1-1
    2. María Florencia Ercoli, Rodrigo Vena, Camila Goldy, Javier F. Palatnik, Ramiro E. Rodríguez
      Pages 3-17
    3. Lijuan Du, Sougata Roy
      Pages 29-45
  3. Analysis of Biophysical Properties and Functions of Morphogens

    1. Front Matter
      Pages 65-65
    2. Xue Wen Ng, Karuna Sampath, Thorsten Wohland
      Pages 67-105
    3. Gary H. Soh, Patrick Müller
      Pages 107-124
    4. Maraysa de Olivera-Melo, Peng-Fei Xu, Nathalie Houssin, Bernard Thisse, Christine Thisse
      Pages 125-141
    5. Holly Stainton, Matthew Towers
      Pages 143-153
    6. Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 155-164
    7. Carmina Angelica Perez-Romero, Huy Tran, Mathieu Coppey, Aleksandra M. Walczak, Cécile Fradin, Nathalie Dostatni
      Pages 165-182
    8. Huy Tran, Carmina Angelica Perez-Romero, Teresa Ferraro, Cécile Fradin, Nathalie Dostatni, Mathieu Coppey et al.
      Pages 183-195
  4. Modeling of Morphogen Behaviors and Activities

    1. Front Matter
      Pages 197-197
    2. Hamid Teimouri, Anatoly B. Kolomeisky
      Pages 199-221
    3. Michael D. Multerer, Lucas D. Wittwer, Anna Stopka, Diana Barac, Christine Lang, Dagmar Iber
      Pages 223-250
    4. Manan’Iarivo Rasolonjanahary, Bakhtier Vasiev
      Pages 263-280
    5. Laurent Jutras-Dubé, Adrien Henry, Paul François
      Pages 281-301
  5. Julien Dubrulle
    Pages E1-E1
  6. Back Matter
    Pages 303-306

About this book


This detailed book presents methods focusing on the visualization of morphogen gradients, the analysis of their biophysical and biological properties, and the theoretical aspects underlying their functions. The study of morphogen gradients combines biophysics, cell and developmental biology, and applied mathematics approaches to understand their formation, their biological functions, and the prediction of their behavior in space and time, and this volume serves as a practical, hands-on guide to the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and helpful, Morphogen Gradients: Methods and Protocols is an ideal collection for researchers in this vital area of study.


Progenitor cells Biophysics Applied mathematics Secreted peptides Metabolites Diffusible transcription factors

Editors and affiliations

  • Julien Dubrulle
    • 1
  1. 1.Integrated Microscopy CoreBaylor College of MedicineHoustonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8771-9
  • Online ISBN 978-1-4939-8772-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site