Programmed Necrosis

Methods and Protocols

  • Adrian T. Ting

Part of the Methods in Molecular Biology book series (MIMB, volume 1857)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Adrian T. Ting
    Pages 1-9
  3. David Mark Moquin, Francis Ka-Ming Chan
    Pages 11-18
  4. Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, Motti Gerlic
    Pages 35-51
  5. Zikou Liu, John Silke, Joanne M. Hildebrand
    Pages 53-61
  6. Diana Legarda, Adrian T. Ting
    Pages 63-70
  7. Diego A. Rodriguez, Douglas R. Green
    Pages 71-83
  8. Zhenyu Cai, Zheng-Gang Liu
    Pages 85-92
  9. Roshan J. Thapa, Shoko Nogusa, Siddharth Balachandran
    Pages 93-99
  10. Allison M. Beal, John Bertin, Michael A. Reilly
    Pages 109-124
  11. Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, Andreas Linkermann
    Pages 135-144
  12. Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, Andreas Linkermann
    Pages 145-151
  13. Joshua D. Webster, Margaret Solon, Susan Haller, Kim Newton
    Pages 153-160
  14. Dario Priem, Yves Dondelinger, Mathieu J. M. Bertrand
    Pages 171-179
  15. Back Matter
    Pages 189-191

About this book


This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.


RIPK1/3 kinases DAMPs alarmins cell death necroptosis

Editors and affiliations

  • Adrian T. Ting
    • 1
  1. 1.Precision Immunology InstituteIcahn School of Medicine at Mount SinaiNew YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8753-5
  • Online ISBN 978-1-4939-8754-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site