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Protein Gel Detection and Imaging

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Pazhani Sundaram
    Pages 1-14
  3. Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, Michael Bachmann
    Pages 27-30
  4. Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, Tian-yun Wang
    Pages 31-35
  5. Nicole Berndt, Ralf Bergmann, Claudia Arndt, Stefanie Koristka, Michael Bachmann
    Pages 47-52
  6. Engelbert Buxbaum
    Pages 87-94
  7. Biji T. Kurien, Yaser Dorri, R. Hal Scofield
    Pages 121-131
  8. K. Divakar, J. Deepa Arul Priya, G. Panneer Selvam, M. Suryia Prabha, Ashwin Kannan, G. Nandhini Devi et al.
    Pages 133-138
  9. Jelena Bogdanović Pristov, Ivan Spasojević
    Pages 149-158
  10. Isamu Kameshita, Noriyuki Sueyoshi, Atsuhiko Ishida
    Pages 165-172
  11. Kazem Asadollahi, Saharnaz Rafiee, Gholamhossein Riazi
    Pages 173-177
  12. Surbhi Desai, Boguslawa R. Dworecki, Marie C. Nlend
    Pages 191-205
  13. Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, Paola Pietrangeli
    Pages 207-221
  14. Anja Feldmann, Nicole Berndt, Ralf Bergmann, Michael Bachmann
    Pages 269-271
  15. Nicole Berndt, Ralf Bergmann, Michael Bachmann
    Pages 281-285
  16. Back Matter
    Pages 287-289

About this book

Introduction

This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.

Keywords

Polyacrylamide Gels Coomassie Blue imidazole-zinc PAGE Metalloproteins

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Section of Endocrinology and DiabetesUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Section of Endocrinology and DiabetesUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8745-0
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8744-3
  • Online ISBN 978-1-4939-8745-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site