Recombinant Protein Expression in Mammalian Cells

Methods and Protocols

  • David L. Hacker

Part of the Methods in Molecular Biology book series (MIMB, volume 1850)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Denis L’Abbé, Louis Bisson, Christian Gervais, Eric Grazzini, Yves Durocher
    Pages 1-16
  3. Joanna Pieprzyk, Samuel Pazicky, Christian Löw
    Pages 17-31
  4. Natascha Gödecke, Hansjörg Hauser, Dagmar Wirth
    Pages 43-55
  5. Cristina Parola, Derek M. Mason, Andreas Zingg, Daniel Neumeier, Sai T. Reddy
    Pages 79-111
  6. David L. Hacker, Laurence Durrer, Soraya Quinche
    Pages 123-131
  7. Sebastian Vogg, Moritz Kai Florian Wolf, Massimo Morbidelli
    Pages 147-178
  8. Tia A. Arena, Peter D. Harms, Athena W. Wong
    Pages 179-187
  9. Kristina Woodruff, Sebastian J. Maerkl
    Pages 189-208
  10. Sarah Inwood, Michael J. Betenbaugh, Madhu Lal, Joseph Shiloach
    Pages 209-219
  11. Kevin Kellner, Ankur Solanki, Thomas Amann, Nga Lao, Niall Barron
    Pages 221-235
  12. Qiong Wang, Cheng-Yu Chung, Julian N. Rosenberg, Geng Yu, Michael J. Betenbaugh
    Pages 237-257
  13. Daniel Blessing, Nicole Déglon, Bernard L. Schneider
    Pages 259-274
  14. Lena Thoring, Stefan Kubick
    Pages 289-308 Open Access
  15. Back Matter
    Pages 309-311

About this book


This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host;  however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via


Transient protein production Recombinant cell lines Chinese hamster ovary cells Human embryonic kidney 293 cells Cell cultivation

Editors and affiliations

  • David L. Hacker
    • 1
  1. 1.Protein Production and Structure Core Facility (PPSCF)Ecole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8729-0
  • Online ISBN 978-1-4939-8730-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site