Plant Pathogenic Fungi and Oomycetes

Methods and Protocols

  • Wenbo Ma
  • Thomas Wolpert

Part of the Methods in Molecular Biology book series (MIMB, volume 1848)

Table of contents

  1. Front Matter
    Pages i-xi
  2. David A. Baltrus, Joseph Spraker, A. Elizabeth Arnold
    Pages 1-11
  3. Mahtab Peyambari, Marilyn J. Roossinck
    Pages 13-24
  4. Shawn P. Brown, Devin R. Leopold, Posy E. Busby
    Pages 39-51
  5. Vidhyavathi Raman, Blake C. Meyers, Ralph A. Dean, Nicole M. Donofrio
    Pages 53-66
  6. Audrey M. V. Ah-Fong, Meenakshi Kagda, Howard S. Judelson
    Pages 119-129
  7. Sidrat Abdullah, Dennis Halterman
    Pages 131-138
  8. Kyle Fletcher, Richard Michelmore
    Pages 151-197
  9. Lanelle R. Connolly, Allyson A. Erlendson, Corinne M. Fargo, Kendra K. Jackson, Morgan M. G. Pelker, Jacob W. Mazzola et al.
    Pages 235-263
  10. Zhiwen Wang, Brett M. Tyler, Xili Liu
    Pages 265-274
  11. Yao Zhao, Yufei Li, Min Qiu, Wenbo Ma, Yuanchao Wang
    Pages 275-286
  12. Back Matter
    Pages 287-292

About this book


This book describes approaches for the analysis of plant pathogenic fungi that range from ecosystem composition to in vitro and wet-lab to computational analyses. Chapters detail bioinformatics protocols for genome assembly, transcriptome analysis and small RNA profiling, characterization of secondary metabolome, analysis of post-translational modification and localization of proteins, examination of virulence function and interaction with plants or other microorganisms, and targeted mutagenesis using various approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through a wide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.


Gene Silencing fungal chromatin Phytophthora Magnaporthe oryzae Arabidopsis

Editors and affiliations

  • Wenbo Ma
    • 1
  • Thomas Wolpert
    • 2
  1. 1.Department of Microbiology and Plant PathologyUniversity of California RiversideRiversideUSA
  2. 2.Oregon State UniversityCorvallisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8723-8
  • Online ISBN 978-1-4939-8724-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site