Methods and Protocols

  • Guillermo Oliver
  • Mark L. Kahn

Part of the Methods in Molecular Biology book series (MIMB, volume 1846)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Cathrin Dierkes, Aaron Scherzinger, Friedemann Kiefer
    Pages 1-18
  3. Ines Martinez-Corral, Taija Makinen
    Pages 37-53
  4. Kazuhide S. Okuda, Sungmin Baek, Benjamin M. Hogan
    Pages 55-70
  5. Tiffany C. Y. Eng, Jonathan W. Astin
    Pages 71-83
  6. Amélie Sabine, Michael J. Davis, Esther Bovay, Tatiana V. Petrova
    Pages 97-129
  7. Sang Heon Suh, Seon Pyo Hong, Intae Park, Joo-Hye Song, Gou Young Koh
    Pages 131-139
  8. Jasmin Herz, Antoine Louveau, Sandro Da Mesquita, Jonathan Kipnis
    Pages 141-151
  9. Benjamin R. Thomson, Susan E. Quaggin
    Pages 153-160
  10. Peter Baluk, Donald M. McDonald
    Pages 161-180
  11. Baptiste Coxam, Holger Gerhardt
    Pages 181-195
  12. Samia B. Bachmann, Michael Detmar, Steven T. Proulx
    Pages 197-211
  13. Daniel T. Sweet, Joshua D. Hall, John Welsh, Mark L. Kahn, Juan M. Jiménez
    Pages 213-227
  14. Jorge A. Castorena-Gonzalez, Joshua P. Scallan, Michael J. Davis
    Pages 229-248
  15. Witold W. Kilarski, Esra Güç, Melody A. Swartz
    Pages 261-277
  16. Sinem Karaman, Harri Nurmi, Salli Antila, Kari Alitalo
    Pages 291-300
  17. Michael Vanlandewijck, Christer Betsholtz
    Pages 309-324
  18. Pengchun Yu, Tiago C. Alves, Richard G. Kibbey, Michael Simons
    Pages 325-334
  19. Matthew T. Menendez, Courtney T. Griffin
    Pages 335-344
  20. Back Matter
    Pages 345-345

About this book


This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.


lymphatic transcriptome endothelial cells stem cells Schlemm’s canal zebrafish

Editors and affiliations

  • Guillermo Oliver
    • 1
  • Mark L. Kahn
    • 2
  1. 1.Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research InstituteNorthwestern UniversityChicagoUSA
  2. 2.Department of Medicine and Cardiovascular InstituteUniversity of PennsylvaniaPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8711-5
  • Online ISBN 978-1-4939-8712-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site