Tertiary Lymphoid Structures

Methods and Protocols

  • Marie-Caroline Dieu-Nosjean

Part of the Methods in Molecular Biology book series (MIMB, volume 1845)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Aaron Silva-Sanchez, Troy D. Randall, Selene Meza-Perez
    Pages 1-15
  3. Christopher George Mueller, Saba Nayar, David Gardner, Francesca Barone
    Pages 17-30
  4. Christophe Klein, Priyanka Devi-Marulkar, Marie-Caroline Dieu-Nosjean, Claire Germain
    Pages 47-69
  5. Karīna Siliņa, Chiara Burkhardt, Ruben Casanova, Alex Solterman, Maries van den Broek
    Pages 71-86
  6. Emma Jones, Awen Gallimore, Ann Ager
    Pages 99-118
  7. Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean, Jérémy Goc
    Pages 119-137
  8. Chunyan Gu-Trantien, Soizic Garaud, Edoardo Migliori, Cinzia Solinas, Jean-Nicolas Lodewyckx, Karen Willard-Gallo
    Pages 139-157
  9. Elisa Corsiero, Lucas Jagemann, Michele Bombardieri, Costantino Pitzalis
    Pages 159-187
  10. Priyanka Devi-Marulkar, Hélène Kaplon, Marie-Caroline Dieu-Nosjean, Myriam Lawand
    Pages 189-204
  11. Coline Couillault, Claire Germain, Bertrand Dubois, Hélène Kaplon
    Pages 205-222
  12. Jean-Luc Teillaud, Lucile Regard, Clémence Martin, Sophie Sibéril, Pierre-Régis Burgel
    Pages 223-239
  13. Anthony B. Rodriguez, J. David Peske, Victor H. Engelhard
    Pages 241-257
  14. Kelli A. Connolly, Mursal Nader, Nikhil Joshi
    Pages 259-273
  15. Haidong Tang, Xiangyan Qiu, Casey Timmerman, Yang-Xin Fu
    Pages 275-286
  16. Back Matter
    Pages 287-289

About this book


This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications.


endothelial venules CD8+ T cells immune function immunotherapy neogenesis cancer research

Editors and affiliations

  • Marie-Caroline Dieu-Nosjean
    • 1
  1. 1.Laboratory “Cancer, Immune Control and Escape”, UMRS 1138 INSERMCordeliers Research Center, Sorbonne University, UMR 1138, Paris Descartes University, Sorbonne Paris City, UMR 1138ParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8708-5
  • Online ISBN 978-1-4939-8709-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site