About this book
This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learning more about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.
cytosolic endoplasmic reticulum Ca2+ Ca2+ influx Ca2+ release-activated Ca2+ current STIM stimulating Orai pore-forming subunits Store-Operated Ca2+ Entry (SOCE)
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-8704-7
- Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-8702-3
- Online ISBN 978-1-4939-8704-7
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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