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The CRAC Channel

Methods and Protocols

  • Aubin Penna
  • Bruno Constantin

Part of the Methods in Molecular Biology book series (MIMB, volume 1843)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald L. Gill, Youjun Wang
    Pages 1-16
  3. Zui Pan, Sangyong Choi, Yanhong Luo
    Pages 55-62
  4. Pedro C. Redondo, Alejandro Berna-Erro, Natalia Dionisio, Juan A. Rosado
    Pages 69-82
  5. Charlotte Dubois, Vyacheslav Lehenkyi, Natalia Prevarskaya
    Pages 95-106
  6. Sumita Chakraborty, Gaiti Hasan
    Pages 125-136
  7. Xuexin Zhang, Amy M. Spinelli, Wei Zhang, Mohamed Trebak
    Pages 137-145
  8. Guolin Ma, Lian He, Ji Jing, Peng Tan, Yun Huang, Yubin Zhou
    Pages 147-166
  9. Andras T. Deak, Benjamin Gottschalk, Emrah Eroglu, Rene Rost, Markus Waldeck-Weiermair, Wolfgang F. Graier et al.
    Pages 175-187
  10. Laura G. Ceballos, Alexander Asanov, Luis Vaca
    Pages 189-201
  11. Back Matter
    Pages 203-204

About this book

Introduction

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learning more about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.

Keywords

cytosolic endoplasmic reticulum Ca2+ Ca2+ influx Ca2+ release-activated Ca2+ current STIM stimulating Orai pore-forming subunits Store-Operated Ca2+ Entry (SOCE)

Editors and affiliations

  • Aubin Penna
    • 1
  • Bruno Constantin
    • 2
  1. 1.IRSET Inserm UMR 1085/ UR1RennesFrance
  2. 2.CNRS ERL 7003Laboratoire STIMPoitiersFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8704-7
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8702-3
  • Online ISBN 978-1-4939-8704-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site