Lipases and Phospholipases

Methods and Protocols

  • Georgina Sandoval

Part of the Methods in Molecular Biology book series (MIMB, volume 1835)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Overview of Lipases, Phospholipases and Esterases

    1. Front Matter
      Pages 1-1
    2. Leticia Casas-Godoy, Francisco Gasteazoro, Sophie Duquesne, Florence Bordes, Alain Marty, Georgina Sandoval
      Pages 3-38
    3. Mariana Armendáriz-Ruiz, Jorge Alberto Rodríguez-González, Rosa María Camacho-Ruíz, Juan Carlos Mateos-Díaz
      Pages 39-68
    4. Ahmed Aloulou, Renaud Rahier, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham
      Pages 69-105
  3. Activity Screening and Expression

    1. Front Matter
      Pages 107-107
    2. Dolores Reyes-Duarte, Cristina Coscolín, Mónica Martínez-Martínez, Manuel Ferrer, Humberto García-Arellano
      Pages 109-117
    3. Meddy El Alaoui, Laurent Soulère, Alexandre Noiriel, Priscila Sutto-Ortiz, Lucie Grand, Florence Popowycz et al.
      Pages 119-128
    4. Renaud Rahier, Houda Abla, Yani Arhab, Alexandre Noiriel, Abdelkarim Abousalham
      Pages 129-138
    5. H. Nolasco-Soria, F. Moyano-López, F. Vega-Villasante, Alberto del Monte-Martínez, D. Espinosa-Chaurand, E. Gisbert et al.
      Pages 139-167
    6. Ivanna Rivera, Abel Gutiérrez-Ortega, Georgina Sandoval
      Pages 169-178
    7. Nabil Smichi, Neila Achouri, Alexandre Noiriel, Vincent Arondel, Abdelkarim Abousalham, Youssef Gargouri et al.
      Pages 179-189
    8. Yani Arhab, Renaud Rahier, Alexandre Noiriel, Mickael V. Cherrier, Abdelkarim Abousalham
      Pages 191-201
  4. Optimization of the Biocatalyst Production and Performances

    1. Front Matter
      Pages 203-203
    2. Doddy Denise Ojeda-Hernández, Ricardo Cosío-Cuadros, Georgina Sandoval, Jorge Alberto Rodríguez-González, Juan Carlos Mateos-Díaz
      Pages 217-228
    3. Heber Gamboa-Melendez, Macarena Larroude, Young Kyoung Park, Pauline Trebul, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro
      Pages 229-242
    4. Alberto del Monte-Martínez, Bessy V. Cutiño-Avila, Jorge González-Bacerio
      Pages 243-283
  5. Applications of Lipases, Phospholipases and Esterases

    1. Front Matter
      Pages 285-285
    2. Jose L. Gonzalez-Alfonso, Leticia Casas-Godoy, Javier Arrizon, Daniel Arrieta-Baez, Antonio O. Ballesteros, Georgina Sandoval et al.
      Pages 287-296
    3. Suzana Ferreira-Dias, Natália M. Osório, Carla Tecelão
      Pages 315-336
    4. Alicia Baldessari, Guadalupe García Liñares
      Pages 337-350
    5. Nadia Guajardo, Pablo Domínguez de María
      Pages 351-357
    6. Marcos Vargas, Xochitl Niehus, Leticia Casas-Godoy, Georgina Sandoval
      Pages 377-390
    7. Georgina Sandoval, Enrique J. Herrera-López
      Pages 391-425
  6. Back Matter
    Pages 427-437

About this book


This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Edition serves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.


Expression systems Plant lipases Transcriptomic Analysis Activity screening Esterases Enzymes Biocatalyst production

Editors and affiliations

  • Georgina Sandoval
    • 1
  1. 1.CIATEJGuadalajaraMexico

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8671-2
  • Online ISBN 978-1-4939-8672-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site