miRNA Biogenesis

Methods and Protocols

  • Ulf Andersson Vang Ørom

Part of the Methods in Molecular Biology book series (MIMB, volume 1823)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Tsung-Cheng Chang, Joshua T. Mendell
    Pages 1-9
  3. Georgios Georgakilas, Nikos Perdikopanis, Artemis G. Hatzigeorgiou
    Pages 11-31
  4. Annita Louloupi, Ulf Andersson Vang Ørom
    Pages 33-41
  5. Ross A. Cordiner, Sara Macias
    Pages 51-62
  6. Annita Louloupi, Ulf Andersson Vang Ørom
    Pages 63-68
  7. Marta Kotlarek, Anna Kubiak, Krystian Jażdżewski, Anna Wójcicka
    Pages 69-85
  8. Monika Kolanowska, Anna Kubiak, Krystian Jażdżewski, Anna Wójcicka
    Pages 87-101
  9. Thomas Treiber, Nora Treiber, Gunter Meister
    Pages 103-114
  10. Madalena M. Reimão-Pinto, Angela M. Rodrigues-Viana, Stefan L. Ameres
    Pages 115-139
  11. Matteo J. Marzi, Francesco Nicassio
    Pages 141-152
  12. James P. Broughton, Amy E. Pasquinelli
    Pages 153-165
  13. Josephine Galipon, Rintaro Ishii, Soh Ishiguro, Yutaka Suzuki, Shinji Kondo, Mariko Okada-Hatakeyama et al.
    Pages 167-183
  14. Parveen Bansal, Ashish Kumar, Sudhir Chandna, Malika Arora, Renu Bansal
    Pages 197-208
  15. Mohammad Farid Zia, Alex S. Flynt
    Pages 209-219
  16. Thomas B. Hansen
    Pages 221-232
  17. Back Matter
    Pages 233-238

About this book


This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic.


microRNA transcription Regulatory small RNAs Therapeutic applications Molecular networks Gene regulation

Editors and affiliations

  • Ulf Andersson Vang Ørom
    • 1
  1. 1.Department of Molecular Biology and GeneticsAarhus UniversityAarhusDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8623-1
  • Online ISBN 978-1-4939-8624-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site