Rho GTPases

Methods and Protocols

  • Francisco Rivero

Part of the Methods in Molecular Biology book series (MIMB, volume 1821)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Computational Modeling and Imaging

    1. Front Matter
      Pages 1-1
    2. Joseph H. R. Hetmanski, Jean-Marc Schwartz, Patrick T. Caswell
      Pages 37-46
    3. Anastasia Zhurikhina, Timothy Qi, Klaus M. Hahn, Timothy C. Elston, Denis Tsygankov
      Pages 47-56
    4. Sara K. Donnelly, Veronika Miskolci, Alice M. Garrastegui, Dianne Cox, Louis Hodgson
      Pages 87-106
    5. Daniel Schlam, Sergio Grinstein, Spencer A. Freeman
      Pages 107-127
  3. Biochemistry and High Throughput Methods

    1. Front Matter
      Pages 129-129
    2. Daniel Brandwein, Junfeng Tong, Laiji Li, Barbara Ballermann, Zhixiang Wang
      Pages 131-140
    3. Peter Simons, Virginie Bondu, Angela Wandinger-Ness, Tione Buranda
      Pages 177-195 Open Access
  4. Cell Types and Processes

    1. Front Matter
      Pages 197-197
    2. Francisco Rivero, Simon Calaminus
      Pages 199-217
    3. Anne Morel, Anne Blangy, Virginie Vives
      Pages 219-233
    4. Natalija Gerasimcik, Lisa S. Westerberg, Eva Severinson
      Pages 235-246
    5. Benjamin Mizukawa, Eric O’Brien, James C. Mulloy, Yi Zheng
      Pages 257-266
    6. Angel J. Santiago-Lopez, Claire-Anne Gutekunst, Robert E. Gross
      Pages 267-281
    7. Jana Schulz, Stefan Schumacher
      Pages 297-317
    8. Lilian C. Russo, Pault Y. Minaya, Luiz E. Silva, Fábio L. Forti
      Pages 319-338
    9. Yuli T. Magalhães, Jessica O. Farias, Lucas F. Monteiro, Fábio L. Forti
      Pages 339-355
  5. Rho GTPases in Non-Mammalian Model Organisms

    1. Front Matter
      Pages 357-357
    2. Nicholas J. Hanovice, Emily McMains, Jeffrey M. Gross
      Pages 359-370
    3. Maja Marinović, Huajiang Xiong, Francisco Rivero, Igor Weber
      Pages 371-392
    4. Yoshihisa Oda, Yoshinobu Nagashima, Hiroo Fukuda
      Pages 393-399
  6. Back Matter
    Pages 411-419

About this book


This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. 

Authoritative and up-to-date, Rho GTPases: Methods and Protocols, Second Edition constitutes an invaluable tool for all those with an interest in this remarkable family of signaling proteins.


Computational modeling Rho signaling dynamics Signaling proteins High throughput methods Pharmacological targets Effectors and regulators

Editors and affiliations

  • Francisco Rivero
    • 1
  1. 1.The Hull York Medical SchoolUniversity of HullHullUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8611-8
  • Online ISBN 978-1-4939-8612-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site