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Mouse Oocyte Development

Methods and Protocols

  • Marie-Hélène Verlhac
  • Marie-Emilie Terret

Part of the Methods in Molecular Biology book series (MIMB, volume 1818)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stephany El-Hayek, Qin Yang, Hugh J. Clarke
    Pages 1-11
  3. Ewa Borsuk, Jacek Z. Kubiak
    Pages 13-21
  4. Jennifer R. Gruhn, Stine Gry Kristensen, Claus Yding Andersen, Eva R. Hoffmann
    Pages 23-30
  5. Louise Hyslop
    Pages 31-36
  6. Agathe Chaigne
    Pages 37-42
  7. Joao P. Sousa Martins, Marco Conti
    Pages 43-50
  8. Maud Borensztein, Laurène Syx, Nicolas Servant, Edith Heard
    Pages 51-65
  9. Jessica Greaney, Zhe Wei, Hayden Homer
    Pages 77-87
  10. Aurélien Courtois, Petr Solc, Tomoya S. Kitajima
    Pages 89-97
  11. Elvira Nikalayevich, Nora Bouftas, Katja Wassmann
    Pages 99-112
  12. Anna Szydłowska, Sabrina Ladstätter, Kikuë Tachibana
    Pages 113-128
  13. Takashi Akera, David M. Chenoweth, Michael A. Lampson
    Pages 129-135
  14. Maria Almonacid
    Pages 145-151
  15. Isma Bennabi, Marion Manil-Ségalen
    Pages 153-161
  16. Janice P. Evans, Douglas N. Robinson
    Pages 163-171
  17. Back Matter
    Pages 173-174

About this book

Introduction

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.



Keywords

Follicle Growth cRNA Meiotic Maturation Photoactivation Photoablation

Editors and affiliations

  • Marie-Hélène Verlhac
    • 1
  • Marie-Emilie Terret
    • 2
  1. 1.CIRBCollège de France, UMR7241, INSERM-U1050ParisFrance
  2. 2.CIRBCollège de France, UMR7241, INSERM-U1050ParisFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8603-3
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8602-6
  • Online ISBN 978-1-4939-8603-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site