Lung Innate Immunity and Inflammation

Methods and Protocols

  • Scott Alper
  • William J. Janssen

Part of the Methods in Molecular Biology book series (MIMB, volume 1809)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Lung Innate, Immunity, and Inflammation

    1. Front Matter
      Pages 1-1
    2. William McKleroy, Kenneth Lyn-Kew
      Pages 3-15
    3. David W. H. Riches, Thomas R. Martin
      Pages 17-30
  3. Isolation and Characterization of Lung Innate Immune Cells

    1. Front Matter
      Pages 31-31
    2. Niki D. J. Ubags, Benjamin T. Suratt
      Pages 45-57
    3. Benjamin L. Edelman, Elizabeth F. Redente
      Pages 59-67
    4. Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok et al.
      Pages 69-82
    5. Beata Kosmider, Robert J. Mason, Karim Bahmed
      Pages 83-90
    6. Di Jiang, Niccolette Schaefer, Hong Wei Chu
      Pages 91-109
    7. Yen-Rei A. Yu, Robert M. Tighe
      Pages 111-119
    8. John Matthew Craig, Neil Raj Aggarwal
      Pages 129-137
    9. Kenneth C. Malcolm
      Pages 139-144
    10. Adrianne L. Stefanski, Dorota S. Raclawska, Christopher M. Evans
      Pages 169-201
    11. Nathan D. Jackson, Lando Ringel, Max A. Seibold
      Pages 203-235
    12. Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, Richard Reisdorph
      Pages 237-262
    13. Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, Richard Reisdorph
      Pages 263-288
    14. Frank Fang-Yao Lee, Scott Alper
      Pages 289-298
  4. Analysis of Lung Innate Immunity and Inflammation

    1. Front Matter
      Pages 299-299
    2. Vandy P. Stober, Stavros Garantziotis
      Pages 301-314
    3. Aneta Gandjeva, Rubin M. Tuder
      Pages 315-329
    4. Yimu Yang, Joseph A. Hippensteel, Eric P. Schmidt
      Pages 331-339
    5. Franco R. D’Alessio
      Pages 341-350
    6. Magdalena M. Gorska
      Pages 351-362
    7. David N. O’Dwyer, Bethany B. Moore
      Pages 363-378
    8. Karina A. Serban, Irina Petrache
      Pages 379-394
    9. Kerry M. Empey, R. Stokes Peebles Jr, William J. Janssen
      Pages 395-414
    10. Dominique N. Price, Pavan Muttil
      Pages 415-429
  5. Back Matter
    Pages 431-433

About this book


This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be aguidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.


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Editors and affiliations

  • Scott Alper
    • 1
  • William J. Janssen
    • 2
  1. 1.Department of Immunology and Microbiology University of Colorado School of MedicineDepartment of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USAAurora,USA
  2. 2.Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado DenverDivision of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USAAuroraUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8569-2
  • Online ISBN 978-1-4939-8570-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site