Immunotoxicity Testing

Methods and Protocols

  • Jamie C. DeWitt
  • Cheryl E. Rockwell
  • Christal C. Bowman

Part of the Methods in Molecular Biology book series (MIMB, volume 1803)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Overview and Health Risk Considerations

    1. Front Matter
      Pages 1-1
    2. Kenneth L. Hastings
      Pages 3-13
    3. L. Peyton Myers
      Pages 15-26
    4. Stacey E. Anderson, Hillary L. Shane
      Pages 27-46
    5. Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, Ellen Evans
      Pages 57-79
  3. Legacy Protocols: In Vivo

    1. Front Matter
      Pages 81-81
    2. Stefanie C. M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, Robert W. Luebke
      Pages 117-145
    3. Sheung P. Ng, Judith T. Zelikoff
      Pages 169-180
  4. Legacy Protocols: Ex Vivo and In Vitro

    1. Front Matter
      Pages 181-181
    2. Scott T. Espenschied, Robert M. Tighe, Kymberly M. Gowdy
      Pages 183-197
    3. Gary R. Burleson, Florence G. Burleson, Rodney R. Dietert
      Pages 199-208
    4. Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, Prakash S. Nagarkatti
      Pages 209-230
    5. Joanna M. Kreitinger, David M. Shepherd
      Pages 243-253
    6. Jennifer Franko, Jamie L. McCall, John B. Barnett
      Pages 255-296
    7. Emanuela Corsini, Robert V. House
      Pages 297-314
  5. Alternative Protocols

    1. Front Matter
      Pages 315-315
    2. Jim Reed, Scott A. Wetzel
      Pages 335-351
  6. Emerging Issues

    1. Front Matter
      Pages 383-383
  7. Back Matter
    Pages 397-412

About this book


This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed.  Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.


Immunotoxicology Immune function Immunomodulatory agents Pharmaceuticals Health risk reduction Xenobiotics

Editors and affiliations

  • Jamie C. DeWitt
    • 1
  • Cheryl E. Rockwell
    • 2
  • Christal C. Bowman
    • 3
  1. 1.Department of Pharmacology and ToxicologyEast Carolina UniversityGreenvilleUSA
  2. 2.Department of Pharmacology and ToxicologyMichigan State UniversityEast LansingUSA
  3. 3.DurhamUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8548-7
  • Online ISBN 978-1-4939-8549-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site