About this book
This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside.
T cell Basophils Mast cells Eosinophils lymphoid cells
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-7896-0
- Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-7895-3
- Online ISBN 978-1-4939-7896-0
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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