Methods and Protocols

  • Mette Lübeck

Part of the Methods in Molecular Biology book series (MIMB, volume 1796)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction Chapter and a Case Example

    1. Front Matter
      Pages 1-1
    2. Sanjeev Kumar Soni, Amita Sharma, Raman Soni
      Pages 3-23
  3. Protocols

    1. Front Matter
      Pages 35-35
    2. Mette Lübeck, Peter Stephensen Lübeck
      Pages 37-45
    3. Yonit Ben-David, Bareket Dassa, Lizi Bensoussan, Edward A. Bayer, Sarah Moraïs
      Pages 67-84
    4. Marta Zoglowek, Heather Brewer, Angela Norbeck
      Pages 103-113
    5. Morgann C. Reilly, Saori Amaike Campen, Blake A. Simmons, Scott E. Baker, John M. Gladden, Jon K. Magnuson
      Pages 123-133
    6. Amaranta Kahn, Edward A. Bayer, Sarah Moraïs
      Pages 135-151
    7. Amaranta Kahn, Anastasia P. Galanopoulou, Dimitris G. Hatzinikolaou, Sarah Moraïs, Edward A. Bayer
      Pages 153-166
  4. Assays for Activity Testing

    1. Front Matter
      Pages 167-167
    2. M. Shafiqur Rahman, Sheran Fernando, Brian Ross, Jiangning Wu, Wensheng Qin
      Pages 169-183
    3. Hem Kanta Sharma, Wensheng Qin, Chunbao (Charles) Xu
      Pages 185-199
    4. Bjørge Westereng, Jennifer S. M. Loose, Gustav Vaaje-Kolstad, Finn L. Aachmann, Morten Sørlie, Vincent G. H. Eijsink
      Pages 219-246
  5. Application of Cellulases for Lignocellulose Degradation

    1. Front Matter
      Pages 271-271
    2. Cristiane S. Farinas, Camila Florencio, Alberto C. Badino
      Pages 273-282
  6. Bioinformatics for Improving Cellulases

    1. Front Matter
      Pages 299-299
    2. Wimal Ubhayasekera
      Pages 301-320
  7. Morgann C. Reilly, Saori Amaike Campen, Blake A. Simmons, Scott E. Baker, John M. Gladden, Jon K. Magnuson
    Pages E1-E1
  8. Back Matter
    Pages 321-324

About this book


This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.


biomass lignocellulose biorefining archea enzyme production

Editors and affiliations

  • Mette Lübeck
    • 1
  1. 1.Section of Sustainable Biotechnology, Department of Chemistry and BioscienceAalborg UniversityCopenhagenDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7876-2
  • Online ISBN 978-1-4939-7877-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site