Methods and Protocols

  • Ashwin Woodhoo

Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Imaging Myelin and Biochemical Analysis

    1. Front Matter
      Pages 1-1
    2. Cinzia Ferri, Angelo Quattrini, Maurizio D’Antonio
      Pages 3-13
    3. M. Thetiot, S. A. Freeman, A. Desmazières
      Pages 15-23
    4. Chunyan Wang, Juan Pablo Palavicini, Xianlin Han
      Pages 37-50
    5. Roberto Fernández, Jone Garate, Beatriz Abad, Begoña Ochoa, José A. Fernández
      Pages 51-65
    6. Akihiro Ishii, David Han, Rashmi Bansal
      Pages 67-77
  3. Cell Culture Systems

    1. Front Matter
      Pages 79-79
    2. Marta Palomo Irigoyen, Miguel Tamayo Caro, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, Ashwin Woodhoo
      Pages 81-93
    3. Maria Victoria Sánchez-Gómez, Mari Paz Serrano, Elena Alberdi, Fernando Pérez-Cerdá, Carlos Matute
      Pages 95-113
    4. Carla Taveggia, Alessandra Bolino
      Pages 115-129
    5. Yi Pang, Kimberly Simpson, José Javier Miguel-Hidalgo, Renate Savich
      Pages 131-144
    6. Amin Sherafat, Robert A. Hill, Akiko Nishiyama
      Pages 145-156
    7. Adrien Vaquié, Alizée Sauvain, Claire Jacob
      Pages 157-168
    8. Nan-Xing Huang, Yun-An A. Shen, Feng Mei
      Pages 169-177
    9. Karen Lariosa-Willingham, Dmitri Leonoudakis
      Pages 179-192
    10. Miguel Tamayo Caro, Marta Palomo Irigoyen, Encarnación Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, Ashwin Woodhoo
      Pages 193-206
  4. Modeling Demyelinating Disorders and In Vivo Targeting Strategies

    1. Front Matter
      Pages 225-225
    2. David G. Gonsalvez, SangWon Yoo, Georgina A. Craig, Rhiannon J. Wood, Jessica L. Fletcher, Simon S. Murray et al.
      Pages 243-250
    3. Gerben Van Hameren, Sergio Gonzalez, Ruani N. Fernando, Claire Perrin-Tricaud, Nicolas Tricaud
      Pages 263-276
    4. Alexia Kagiava, Kleopas A. Kleopa
      Pages 277-285
  5. Back Matter
    Pages 293-294

About this book


This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.


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Editors and affiliations

  • Ashwin Woodhoo
    • 1
    • 2
  1. 1.Nerve Disorders LaboratoryCIC bioGUNEDerioSpain
  2. 2.Basque Foundation for ScienceIKERBASQUEBilbaoSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7861-8
  • Online ISBN 978-1-4939-7862-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site