Plant Vacuolar Trafficking

Methods and Protocols

  • Cláudia Pereira

Part of the Methods in Molecular Biology book series (MIMB, volume 1789)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Tomoo Shimada, Kentaro Fuji, Takuji Ichino, Ooi-Kock Teh, Yasuko Koumoto, Ikuko Hara-Nishimura
    Pages 1-7
  3. Bruno Peixoto, Susana Pereira, Cláudia Pereira, José Pissarra
    Pages 21-31
  4. Inês Carqueijeiro, Liuda J. Sepúlveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon et al.
    Pages 33-54
  5. Giovanni Stefano, Luciana Renna, Federica Brandizzi
    Pages 55-63
  6. Carolina Gabriela Ocampo, Silvana Petruccelli
    Pages 65-80
  7. Inês Carqueijeiro, Henrique Noronha, Sara Bettencourt, Joana G. Guedes, Patrícia Duarte, Hernâni Gerós et al.
    Pages 81-99
  8. Jorge M. P. Rodrigues, Cátia S. Pereira, Natacha Fontes, Hernâni Gerós, Manuela Côrte-Real
    Pages 101-115
  9. Luciana Renna, Giovanni Stefano, Federica Brandizzi
    Pages 117-130
  10. Alexandra Chanoca, Brian Burkel, Erich Grotewold, Kevin W. Eliceiri, Marisa S. Otegui
    Pages 131-141
  11. Ricardo Tejos, Claudio Osorio-Navarro, Lorena Norambuena
    Pages 143-154
  12. Back Matter
    Pages 221-222

About this book


This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field


fusion proteins vacuoles Immunofluorescence spinning disk imaging FLIM

Editors and affiliations

  • Cláudia Pereira
    • 1
  1. 1.Faculty of SciencesUniversity of PortoPortoPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7855-7
  • Online ISBN 978-1-4939-7856-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site