Epitope Mapping Protocols

  • Johan Rockberg
  • Johan Nilvebrant

Part of the Methods in Molecular Biology book series (MIMB, volume 1785)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Johan Nilvebrant, Johan Rockberg
    Pages 1-10
  3. Whole Antigen Methods

    1. Front Matter
      Pages 11-11
    2. Luca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, Luca Varani
      Pages 29-51
    3. Diluka Peiris, Teodor Aastrup, Samuel Altun, Camilla Käck, Maria Gianneli, Davide Proverbio et al.
      Pages 53-63
    4. Thomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton et al.
      Pages 89-118
  4. Protein Domain and Fragment-Based Methods

    1. Front Matter
      Pages 119-119
    2. Thiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, Michael W. Heuzenroeder
      Pages 121-128
    3. Urpo Lamminmäki, Gaurav Batra, Petri Saviranta
      Pages 129-140
    4. Anna-Luisa Volk, Francis Jingxin Hu, Johan Rockberg
      Pages 141-157
  5. Real-Time Based Methods

  6. Antibody Specificity and T-Cell Prediction

    1. Front Matter
      Pages 221-221
    2. Pedro Ramos-López, José Irizarry, Ignacio Pino, Seth Blackshaw
      Pages 223-229
    3. Ronald Sjöberg, Eni Andersson, Cecilia Hellström, Cecilia Mattsson, Jochen M. Schwenk, Peter Nilsson et al.
      Pages 231-238
    4. Burcu Ayoglu, Peter Nilsson, Jochen M. Schwenk
      Pages 239-248
    5. John T. Ballew, Jack R. Reifert, Patrick S. Daugherty
      Pages 249-268
    6. Massimo Andreatta, Morten Nielsen
      Pages 269-281
  7. Back Matter
    Pages 283-284

About this book


This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.


antibodies antigens protein-protein interactions nuclear magnetic resonance (NMR) DNA sequencing ELISA t-cell epitope

Editors and affiliations

  • Johan Rockberg
    • 1
  • Johan Nilvebrant
    • 2
  1. 1.KTH School of Engineering Sciences in Chemistry, Biotechnology and HealthProtein TechnologyStockholmSweden
  2. 2.Donnelly Centre for Cellular and Biomolecular ResearchUniversity of TorontoTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7839-7
  • Online ISBN 978-1-4939-7841-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site