Mitochondrial Bioenergetics

Methods and Protocols

  • Carlos M. Palmeira
  • António J. Moreno

Part of the Methods in Molecular Biology book series (MIMB, volume 1782)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Vitor M. C. Madeira
    Pages 1-6
  3. Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, Erich Gnaiger
    Pages 31-70
  4. James Hynes, Rachel L. Swiss, Yvonne Will
    Pages 71-87
  5. João Soeiro Teodoro, Carlos Marques Palmeira, Anabela Pinto Rolo
    Pages 109-119
  6. Timea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier et al.
    Pages 137-155
  7. Charles Affourtit, Hoi-Shan Wong, Martin D. Brand
    Pages 157-170
  8. Randi J. Parks, Elizabeth Murphy, Julia C. Liu
    Pages 187-196
  9. James R. Roede, Young-Mi Go, Dean P. Jones
    Pages 197-227
  10. Rui A. Carvalho, Ivana Jarak
    Pages 229-247
  11. Sonia Cortassa, Steven J. Sollott, Miguel A. Aon
    Pages 249-265
  12. Luca Simula, Silvia Campello
    Pages 267-285
  13. Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, Martin D. Brand
    Pages 287-299
  14. Shona A. Mookerjee, Casey L. Quinlan, Hoi-Shan Wong, Pratiksha Dighe, Martin D. Brand
    Pages 301-313
  15. Shun-ichi Yamashita, Tomotake Kanki
    Pages 315-324
  16. Venkat K. Ramshesh, John J. Lemasters
    Pages 351-356
  17. Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, Mariusz R. Wieckowski
    Pages 357-381
  18. Felix T. Kurz, Miguel A. Aon, Brian O’Rourke, Antonis A. Armoundas
    Pages 383-402
  19. Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, Fabio Di Lisa
    Pages 403-418
  20. Back Matter
    Pages 419-422

About this book


This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.


OXPHOS Phosphorylation Proton Leak Calcium Signalling mitochondrial regulation

Editors and affiliations

  • Carlos M. Palmeira
    • 1
  • António J. Moreno
    • 2
  1. 1.Department of Life SciencesUniversity of CoimbraCoimbraPortugal
  2. 2.Department of Life SciencesUniversity of CoimbraCoimbraPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7830-4
  • Online ISBN 978-1-4939-7831-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site