Plant Metabolomics

Methods and Protocols

  • Carla António

Part of the Methods in Molecular Biology book series (MIMB, volume 1778)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Review Chapters

    1. Front Matter
      Pages 1-1
    2. Marina C. M. Martins, Camila Caldana, Lucia Daniela Wolf, Luis Guilherme Furlan de Abreu
      Pages 3-17
    3. Valéria F. Lima, Leonardo Perez de Souza, Thomas C. R. Williams, Alisdair R. Fernie, Danilo M. Daloso
      Pages 47-58
    4. Leonardo Perez de Souza, Alisdair R. Fernie, Takayuki Tohge
      Pages 59-67
  3. Mass Spectrometry-Based Methodology Chapters

    1. Front Matter
      Pages 69-69
    2. Regina Feil, John Edward Lunn
      Pages 87-100
    3. Camille Bénard, Sébastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon et al.
      Pages 101-124
    4. Thusitha W. T. Rupasinghe, Ute Roessner
      Pages 125-135
    5. Yozo Okazaki, Kazuki Saito
      Pages 157-169
    6. Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, Takayuki Tohge
      Pages 171-181
    7. Ondřej Novák, Kristýna Floková
      Pages 183-192
    8. Nirupama Samanmalie Jayasinghe, Himasha Mendis, Ute Roessner, Daniel Anthony Dias
      Pages 207-223
    9. José G. Vallarino, Alexander Erban, Ines Fehrle, Alisdair R. Fernie, Joachim Kopka, Sonia Osorio
      Pages 225-239
    10. Berin A. Boughton, Dinaiz Thinagaran
      Pages 241-252
    11. Desalegn W. Etalo, Carmen Díez-Simón, Ric C. H. de Vos, Robert D. Hall
      Pages 253-267
    12. Keiko Masuda, Yasmine Abouleila, Ahmed Ali, Toshio Yanagida, Tsutomu Masujima
      Pages 269-282
  4. Data Analysis & Statistics Chapters

    1. Front Matter
      Pages 283-283
    2. Nadine Töpfer, Samuel M. D. Seaver, Asaph Aharoni
      Pages 297-310
    3. Francisco de Abreu e Lima, Lydia Leifels, Zoran Nikoloski
      Pages 321-327
    4. Lisa Fürtauer, Jakob Weiszmann, Wolfram Weckwerth, Thomas Nägele
      Pages 329-347
  5. Back Matter
    Pages 349-355

About this book


This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field.  The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.


Mass spectrometry Data analysis Plant biological samples Liquid chromatography Primary and secondary metabolites Bioinformatics

Editors and affiliations

  • Carla António
    • 1
  1. 1.Plant Metabolomics LaboratoryInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)OeirasPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7818-2
  • Online ISBN 978-1-4939-7819-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site