Amyloid Proteins

Methods and Protocols

  • Einar M. Sigurdsson
  • Miguel Calero
  • María Gasset

Part of the Methods in Molecular Biology book series (MIMB, volume 1779)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. In Vitro Models and Assays

    1. Front Matter
      Pages 1-1
    2. Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, David B. Teplow
      Pages 3-12
    3. Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, Natàlia Carulla
      Pages 13-22
    4. Agueda Rostagno, Thomas A. Neubert, Jorge Ghiso
      Pages 23-43
    5. Leire Almandoz-Gil, Martin Ingelsson, Joakim Bergström
      Pages 61-71
    6. Istvan Horvath, Sandra Rocha, Pernilla Wittung-Stafshede
      Pages 73-83
    7. Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, Ottavio Arancio
      Pages 85-97
    8. Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, Eckhard Mandelkow
      Pages 99-111
    9. Urmi Sengupta, Mariana Carretero-Murillo, Rakez Kayed
      Pages 113-146
    10. Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset, Cláudio M. Gomes
      Pages 167-179
    11. Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles
      Pages 181-196
    12. Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, Maria Gasset
      Pages 197-207
    13. Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, Bente Vestergaard
      Pages 209-239
  3. Cell Culture Models and Assays

    1. Front Matter
      Pages 265-265
    2. Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, Matthew R. Chapman
      Pages 267-288
    3. Laura Molina-García, Fátima Gasset-Rosa, María Moreno-del Álamo, Susana Moreno-Díaz de la Espina, Rafael Giraldo
      Pages 289-312
    4. Reed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, Herman K. Edskes
      Pages 313-339
    5. Lucía Núñez, María Calvo-Rodríguez, Erica Caballero, Mónica García-Durillo, Carlos Villalobos
      Pages 341-354
    6. Finnbogi R. Thormodsson, Ingvar H. Olafsson, Dadi Th. Vilhjalmsson
      Pages 355-369
    7. Dov B. Shamir, Yan Deng, Einar M. Sigurdsson
      Pages 371-379
    8. Adela Bernabeu-Zornoza, Raquel Coronel, María Lachgar, Charlotte Palmer, Isabel Liste
      Pages 381-398
  4. In Vivo Models and Assays

    1. Front Matter
      Pages 399-399
    2. Shi Fang Yan, Firoz Akhter, Alexander A. Sosunov, Shirley ShiDu Yan
      Pages 415-433
    3. Mar Pérez, Raquel Cuadros, Miguel Medina
      Pages 447-461
    4. Sergio Veiga, Judith Wahrheit, Andrés Rodríguez-Martín, Denise Sonntag
      Pages 463-470
    5. Aidan Kenny, Eva M. Jimenez-Mateos, Miguel Calero, Miguel Medina, Tobias Engel
      Pages 471-484
    6. Alejandra Kun, Fernando González-Camacho, Silvia Hernández, Alexandra Moreno-García, Olga Calero, Miguel Calero
      Pages 497-512
    7. Senthilkumar Krishnaswamy, Qian Wu, Yan Lin, Wajitha J. Rajamohamedsait, Hameetha B. Rajamohamedsait, Einar M. Sigurdsson
      Pages 513-526
    8. Anne Bertrand, Maria Baron, Dung M. Hoang, Lindsay K. Hill, Sebastian L. Mendoza, Einar M. Sigurdsson et al.
      Pages 527-541
  5. Back Matter
    Pages 543-547

About this book


This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety  of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.


Fibrillogenesis Quartz Crystal Fibre Diffraction Fluorescence Microscopy Affinity Chromatography

Editors and affiliations

  • Einar M. Sigurdsson
    • 1
  • Miguel Calero
    • 2
  • María Gasset
    • 3
  1. 1.Department of Neuroscience and PhysiologyNew York University School of Medicine, New York University Langone HealthNew YorkUSA
  2. 2.Chronic Disease Programme-CROSADIS CIBERNED, Queen Sofia FoundationAlzheimer Center CIEN Foundation, Instituto de Salud Carlos IIIMadridSpain
  3. 3.Institute of Physical Chemistry “Rocasolano”Spanish National Research Council (CSIC)MadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7815-1
  • Online ISBN 978-1-4939-7816-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site