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Planarian Regeneration

Methods and Protocols

  • Jochen C. Rink

Part of the Methods in Molecular Biology book series (MIMB, volume 1774)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ronald Sluys, Marta Riutort
    Pages 1-56
  3. Miquel Vila-Farré, Jochen C. Rink
    Pages 173-205
  4. M. Shane Merryman, Alejandro Sánchez Alvarado, James C. Jenkin
    Pages 241-258
  5. Shang-Yun Liu, Jochen C. Rink
    Pages 259-265
  6. Markus A. Grohme, Miquel Vila-Farré, Jochen C. Rink
    Pages 267-275
  7. Shasha Zhang, Alejandro Sánchez Alvarado
    Pages 277-284
  8. Leigh Winsor, Ronald Sluys
    Pages 285-351
  9. David J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, Ricardo M. Zayas
    Pages 353-366
  10. Teresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, Francesc Cebrià
    Pages 367-378
  11. Ryan S. King, Phillip A. Newmark
    Pages 379-392
  12. Li-Chun Cheng, Alejandro Sánchez Alvarado
    Pages 423-434
  13. Brad Stubenhaus, Jason Pellettieri
    Pages 435-444
  14. Carolyn E. Adler, Alejandro Sánchez Alvarado
    Pages 445-454
  15. Irving E. Wang, Daniel E. Wagner, Peter W. Reddien
    Pages 479-495
  16. José I. Rojo-Laguna, Emili Saló
    Pages 497-505
  17. Wei Shen, Yun Shen, Yun Wah Lam, Danny Chan
    Pages 507-518
  18. Alexander Böser, Hannes C. A. Drexler, Kerstin Bartscherer
    Pages 539-553
  19. Back Matter
    Pages 571-573

About this book

Introduction

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Keywords

pluripotent adult stem cells planarian ecology RNA extraction DNA extraction single stem cell transplantations electron microscopy metabolomics analysis model organisms

Editors and affiliations

  • Jochen C. Rink
    • 1
  1. 1.Max Planck Institute of Molecular Cell Biology and GeneticsDresdenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7802-1
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7800-7
  • Online ISBN 978-1-4939-7802-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site