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Reporter Gene Assays

Methods and Protocols

  • Robert Damoiseaux
  • Samuel Hasson

Part of the Methods in Molecular Biology book series (MIMB, volume 1755)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Patricia Dranchak, John J. Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, John Svaren
    Pages 1-17
  3. Ellen Siebring-van Olst, Victor W. van Beusechem
    Pages 19-29
  4. Chidambaram Ramanathan, Andrew C. Liu
    Pages 49-64
  5. Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 89-105
  6. Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 107-120
  7. Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 121-133
  8. Crysten E. Blaby-Haas, M. Dudley Page, Sabeeha S. Merchant
    Pages 135-148
  9. Yaping Liu, Jeffrey Hermes, Jing Li, Matthew Tudor
    Pages 163-177
  10. Erica Cook, Jeffrey Hermes, Jing Li, Matthew Tudor
    Pages 179-195
  11. Sean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. Mcnutt, Anna Coulon Spektor et al.
    Pages 197-221
  12. Nathan Y. Bentolila, Raymond L. Barnhill, Claire Lugassy, Laurent A. Bentolila
    Pages 223-232
  13. Back Matter
    Pages 233-234

About this book

Introduction

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.

Keywords

genes gene expression patterns cellular functions NFkB activation in immune cells high-throughput drug screening pathogenic protein expression

Editors and affiliations

  • Robert Damoiseaux
    • 1
  • Samuel Hasson
    • 2
  1. 1.Department of Molecular and Medical Pharmacology, David Geffen School of MedicineUniversity of California Los AngelesLos AngelesUSA
  2. 2.Pfizer, Inc.CambridgeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7724-6
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7722-2
  • Online ISBN 978-1-4939-7724-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site