Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1747)
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Table of contents (22 protocols)
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Back Matter
About this book
This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.
Editors and Affiliations
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Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany
Alexander Mengel, Christian Lindermayr
Bibliographic Information
Book Title: Nitric Oxide
Book Subtitle: Methods and Protocols
Editors: Alexander Mengel, Christian Lindermayr
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7695-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-7694-2Published: 30 March 2018
Softcover ISBN: 978-1-4939-9260-7Published: 10 December 2019
eBook ISBN: 978-1-4939-7695-9Published: 30 March 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 302
Number of Illustrations: 22 b/w illustrations, 45 illustrations in colour
Topics: Biochemistry, general