Extracellular RNA

Methods and Protocols

  • Tushar Patel

Part of the Methods in Molecular Biology book series (MIMB, volume 1740)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler et al.
    Pages 1-15
  3. Julia Small, Sabrina Roy, Roger Alexander, Leonora Balaj
    Pages 17-21
  4. Sawen Bakr, Bridget Simonson, Kirsty M. Danielson, Saumya Das
    Pages 23-34
  5. Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar Patel
    Pages 35-41
  6. Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang et al.
    Pages 43-57
  7. Irene K. Yan, Valentin X. Berdah, Tushar Patel
    Pages 59-67
  8. Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, Joshua N. Leonard
    Pages 109-124
  9. Juan Pablo Tosar, Alfonso Cayota
    Pages 125-137
  10. Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, Robert L. Raffai
    Pages 139-153
  11. Irene K. Yan, Rishabh Lohray, Tushar Patel
    Pages 155-162
  12. Alton Etheridge, Kai Wang, David Baxter, David Galas
    Pages 163-175
  13. Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, Anastasia Khvorova
    Pages 199-214
  14. Back Matter
    Pages 215-216

About this book


This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field. 


biomarkers exRNA Nanosight RNA isolation density gradient ultracentrifugation Nanostring

Editors and affiliations

  • Tushar Patel
    • 1
  1. 1.Department of TransplantationMayo ClinicJacksonvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7651-5
  • Online ISBN 978-1-4939-7652-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site