Bacterial Chemosensing

Methods and Protocols

  • Michael D. Manson

Part of the Methods in Molecular Biology book series (MIMB, volume 1729)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Michael D. Manson
      Pages 3-6
  3. Methods for Studying Bacterial Chemotaxis at the Population and Whole-Cell Levels

    1. Front Matter
      Pages 21-21
    2. Benjamin A. Webb, Timofey D. Arapov, Birgit E. Scharf
      Pages 33-45
    3. Nitesh Sule, Daniel Penarete-Acosta, Derek L. Englert, Arul Jayaraman
      Pages 47-59
    4. Linda Turner, Howard C. Berg
      Pages 71-76
  4. In Vivo Analysis of Receptor Function

    1. Front Matter
      Pages 77-77
    2. Hanna E. Walukiewicz, George W. Ordal, Christopher V. Rao
      Pages 95-105
    3. Anja Paulick, Victor Sourjik
      Pages 107-126
    4. Rahmi Yusuf, Robert J. Lawrence, Lucy V. Eke, Roger R. Draheim
      Pages 147-158
  5. Cryo-EM Methods for Studying Chemoreceptor Structure

  6. Monitoring the Intracellular Movement of Chemosensory Proteins

    1. Front Matter
      Pages 201-201
    2. Jacopo Solari, Francois Anquez, Katharina M. Scherer, Thomas S. Shimizu
      Pages 203-231
  7. High-Throughput Methods for Screening Novel Chemoeffectors

    1. Front Matter
      Pages 253-253
    2. Rebecca E. Parales, Jayna L. Ditty
      Pages 255-280
    3. Maximilian K. G. Ehrhardt, Suzanne L. Warring, Monica L. Gerth
      Pages 281-290
    4. Matilde Fernández, Álvaro Ortega, Miriam Rico-Jiménez, David Martín-Mora, Abdelali Daddaoua, Miguel A. Matilla et al.
      Pages 291-301
  8. Creating Chemical Tools for Studying Chemosensory Signal Transduction

    1. Front Matter
      Pages 319-319
    2. Daniel B. Lookadoo, Matthew S. Beyersdorf, Christopher J. Halkides
      Pages 337-343
  9. Computer Simulation of Chemotaxis

    1. Front Matter
      Pages 345-345
    2. Asuka A. Orr, Arul Jayaraman, Phanourios Tamamis
      Pages 353-372
    3. Davi R. Ortega, Igor B. Zhulin
      Pages 373-385
  10. Back Matter
    Pages 387-389

About this book


This volume covers a wide range of up-to-date technologies that have been successfully applied to study the chemosensing behavior of the traditional model species, such as Escherichia coli and Salmonella typhimurium, while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis of receptor function, cryo-EM methods for studying chemoreceptor structure, as well as intracellular movement of chemosensory proteins, high-throughput methods to screen for novel chemoeffectors, and chemical tools and computer simulations for analyzing chemotaxis. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Bacterial Chemosensing: Methods and Protocols provides an extensive repertoire of approaches that can be extended to understanding chemotaxis, in particular, and chemosensing, in general, in the context of the enormously varied lifestyles adopted in the larger bacterial world.


Chemoreceptor Chemotaxis Chemosensory behavior Signal transduction system Cryo-electron tomography Intracellular movement

Editors and affiliations

  • Michael D. Manson
    • 1
  1. 1.Department of BiologyTexas A&M UniversityCollege StationUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7576-1
  • Online ISBN 978-1-4939-7577-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site