Noncanonical Amino Acids

Methods and Protocols

  • Edward A. Lemke

Part of the Methods in Molecular Biology book series (MIMB, volume 1728)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Engineering in the Test Tube

    1. Front Matter
      Pages 1-1
    2. Jonas Helma, Heinrich Leonhardt, Christian P. R. Hackenberger, Dominik Schumacher
      Pages 67-93
  3. Engineering in Prokaryotes

  4. Engineering in Eukaryotes

    1. Front Matter
      Pages 219-219
    2. Simon J. Elsässer
      Pages 237-245
    3. Christian Hoffmann, Heinz Neumann, Petra Neumann-Staubitz
      Pages 247-262
    4. Eszter Kozma, Giulia Paci, Gemma Estrada Girona, Edward A. Lemke, Péter Kele
      Pages 337-363
    5. Lloyd Davis, Sebastian Greiss
      Pages 389-408
  5. Back Matter
    Pages 409-411

About this book


This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application.  Chapters have been divided into three parts describing methods for protein production in the test tube, in prokaryotes, and in eukaryotes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.


E. coli Drosophila Xenopus tRNA TAG

Editors and affiliations

  • Edward A. Lemke
    • 1
  1. 1.Structural and Computational Biology Unit & Cell Biology and Biophysics UnitEMBLHeidelbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7573-0
  • Online ISBN 978-1-4939-7574-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site