Neurotrophic Factors

Methods and Protocols

  • Stephen D. Skaper

Part of the Methods in Molecular Biology book series (MIMB, volume 1727)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Stephen D. Skaper
    Pages 1-17
  3. Laura Facci, Stephen D. Skaper
    Pages 39-47
  4. Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, Laura Facci
    Pages 107-118
  5. Laura Facci, Massimo Barbierato, Stephen D. Skaper
    Pages 127-137
  6. Cinzia Volonté, Adele De Ninno, Susanna Amadio
    Pages 139-154
  7. Massimo Barbierato, Stephen D. Skaper, Laura Facci
    Pages 155-166
  8. M. Foster Olive, Armani P. Del Franco, Cassandra D. Gipson
    Pages 179-200
  9. Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, Tomoyuki Takahashi
    Pages 201-215
  10. Matias Alloatti, Luciana Bruno, Tomas L. Falzone
    Pages 217-226
  11. Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny et al.
    Pages 239-250
  12. Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes
    Pages 251-259

About this book


This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology.


Neurobiological research Animal models Cell vitality Neurons and glia Neurotrophin receptors Nervous system pathology

Editors and affiliations

  • Stephen D. Skaper
    • 1
  1. 1.Department of Pharmaceutical and Pharmacological SciencesUniversity of PadovaPadovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7570-9
  • Online ISBN 978-1-4939-7571-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book