Laser Capture Microdissection

Methods and Protocols

  • Graeme I. Murray

Part of the Methods in Molecular Biology book series (MIMB, volume 1723)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, Edwin De Pauw
    Pages 19-31
  3. DaRue A. Prieto, Gordon Whitely, Donald J. Johann Jr., Josip Blonder
    Pages 95-110
  4. Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio et al.
    Pages 139-154
  5. Michele Cummings, Georgia Mappa, Nicolas M. Orsi
    Pages 155-166
  6. Sarah A. Mauney, Tsung-Ung W. Woo, Kai C. Sonntag
    Pages 203-221
  7. Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, David A. Laplaud
    Pages 261-271
  8. Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, Eduardo Molina-Holgado
    Pages 285-318
  9. Corinna Rosenbaum, Martina Böttner, Thilo Wedel, Marco Metzger
    Pages 361-369
  10. David C. Hondius, Jeroen J. M. Hoozemans, Annemieke J. M. Rozemuller, Ka Wan Li, August B. Smit
    Pages 371-383
  11. Ronny Brandt, Martin Mascher, Johannes Thiel
    Pages 397-409
  12. Back Matter
    Pages 411-412

About this book


This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.


Pathology Tissue-based laser microdissection Next generation sequencing Gene expression analysis Microarrays Tissue samples

Editors and affiliations

  • Graeme I. Murray
    • 1
  1. 1.School of Medicine, Medical Sciences and NutritionUniversity of AberdeenAberdeenUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7557-0
  • Online ISBN 978-1-4939-7558-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site