mRNA Decay

Methods and Protocols

  • Shireen R. Lamandé

Part of the Methods in Molecular Biology book series (MIMB, volume 1720)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Toshimichi Yamada, Naoto Imamachi, Rena Onoguchi-Mizutani, Katsutoshi Imamura, Yutaka Suzuki, Nobuyoshi Akimitsu
    Pages 1-13
  3. Tatjana Trcek, Samir Rahman, Daniel Zenklusen
    Pages 35-54
  4. Aitor Garzia, Pavel Morozov, Marcin Sajek, Cindy Meyer, Thomas Tuschl
    Pages 55-75
  5. Bridlin Barckmann, Jérémy Dufourt, Martine Simonelig
    Pages 89-110
  6. Rebecca Petri, Johan Jakobsson
    Pages 131-140
  7. Thomas D. Baird, J. Robert Hogg
    Pages 161-173
  8. Nadezhda M. Markina, Anton P. Pereverzev, Dmitry B. Staroverov, Konstantin A. Lukyanov, Nadya G. Gurskaya
    Pages 187-204
  9. Back Matter
    Pages 225-227

About this book

Introduction

This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.

Keywords

Decay pathways mRNA-binding protein targets Metabolic labeling RNAseq Bioinformatics Nonsense-mediated mRNA decay Tissue homeostasis regulation Genome-wide sequencing

Editors and affiliations

  • Shireen R. Lamandé
    • 1
  1. 1.Murdoch Children’s Research InstituteThe Royal Children’s HospitalParkvilleAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7540-2
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7539-6
  • Online ISBN 978-1-4939-7540-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book