Next Generation Sequencing

Methods and Protocols

  • Steven R. Head
  • Phillip Ordoukhanian
  • Daniel R. Salomon

Part of the Methods in Molecular Biology book series (MIMB, volume 1712)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Fei Xavier Chen, Stacy A. Marshall, Yu Deng, Sun Tianjiao
    Pages 19-26
  3. Lieselot Deleye, Dieter De Coninck, Dieter Deforce, Filip Van Nieuwerburgh
    Pages 27-42
  4. Bernard J. Pope, Fleur Hammet, Tu Nguyen-Dumont, Daniel J. Park
    Pages 53-70
  5. Robert M. Bowers, Janey Lee, Tanja Woyke
    Pages 97-111
  6. Phillip A. Morin, Andrew D. Foote, Christopher M. Hill, Benoit Simon-Bouhet, Aimee R. Lang, Marie Louis
    Pages 113-144
  7. Casey P. Shannon, Chen Xi Yang, Scott J. Tebbutt
    Pages 175-201
  8. Edwin H. Yau, Tariq M. Rana
    Pages 203-216
  9. Marie Holt, Anne Costanzo, Louis Gioia, Brian Abe, Andrew I. Su, Luc Teyton
    Pages 217-238
  10. Xiangzhi Meng, Nicole Riley, Ryan Thompson, Siddhartha Sharma
    Pages 239-252
  11. Back Matter
    Pages 263-266

About this book


This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,  method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field. 


ChIPseq DNA Sequencing NGS RNA Sequencing high throughput sequencing

Editors and affiliations

  • Steven R. Head
    • 1
  • Phillip Ordoukhanian
    • 2
  • Daniel R. Salomon
    • 3
  1. 1.Next Generation Sequencing and Microarray Core FacilityThe Scripps Research InstituteLa JollaUSA
  2. 2.Next Generation Sequencing and Microarray Core FacilityThe Scripps Research InstituteLa JollaUSA
  3. 3.Department of Molecular MedicineThe Scripps Research Institute Center for Organ and Cell TransplantationLa JollaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7512-9
  • Online ISBN 978-1-4939-7514-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site