Methods and Protocols

  • Stuart K. Calderwood
  • Thomas L. Prince

Part of the Methods in Molecular Biology book series (MIMB, volume 1709)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, Nahid F. Mivechi
    Pages 1-22
  3. Ayesha Murshid, Thomas L. Prince, Ben Lang, Stuart K. Calderwood
    Pages 23-34
  4. Toshiki Kijima, Takanori Eguchi, Len Neckers, Thomas L. Prince
    Pages 35-45
  5. Jason Davenport, Lakshmi Galam, Robert L. Matts
    Pages 87-96
  6. Sheah Lin Lee, Nina Claire Dempsey-Hibbert, Dale Vimalachandran, Terence David Wardle, Paul A. Sutton, John H. H. Williams
    Pages 97-105
  7. Alexander E. Kabakov, Vladimir L. Gabai
    Pages 107-127
  8. Sudhakar Voruganti, Jake T. Kline, Maurie J. Balch, Janet Rogers, Robert L. Matts, Steven D. Hartson
    Pages 139-162
  9. Heike Rampelt, Matthias P. Mayer, Bernd Bukau
    Pages 179-188
  10. Joel R. Hoskins, Sue Wickner, Shannon M. Doyle
    Pages 199-207
  11. Rebecca A. Sager, Mark R. Woodford, Len Neckers, Mehdi Mollapour
    Pages 209-219
  12. Aneliya Yoveva, Ritwick Sawarkar
    Pages 221-231
  13. Benjamin J. Lang, Kristina M. Holton, Jianlin Gong, Stuart K. Calderwood
    Pages 233-252

About this book


This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.  Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.


proteome protein folding heat shock proteins transcription factors mouse model

Editors and affiliations

  • Stuart K. Calderwood
    • 1
  • Thomas L. Prince
    • 2
  1. 1.Department of RadiationOncology, Beth Israel Deaconess Medical Center, Harvard Medical SchoolBostonUSA
  2. 2.Department of UrologyWeis Center for Research, Geisinger ClinicDanvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7476-4
  • Online ISBN 978-1-4939-7477-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book