B Cell Receptor Signaling

Methods and Protocols

  • Chaohong Liu

Part of the Methods in Molecular Biology book series (MIMB, volume 1707)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, Haiying Hang
    Pages 1-14
  3. Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, Lilin Ye
    Pages 15-38
  4. Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, Arpita Upadhyaya
    Pages 51-68
  5. Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, Alexander Hoffmann
    Pages 81-94
  6. Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li et al.
    Pages 95-109
  7. Anurag R. Mishra, Akanksha Chaturvedi
    Pages 121-129
  8. Jia C. Wang, Madison Bolger-Munro, Michael R. Gold
    Pages 131-161
  9. Kathrin Kläsener, Jianying Yang, Michael Reth
    Pages 171-181
  10. Laabiah Wasim, Bebhinn Treanor
    Pages 183-192
  11. Vid Šuštar, Marika Vainio, Pieta K. Mattila
    Pages 243-257
  12. Back Matter
    Pages 259-262

About this book


This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field.


Drosophila S2 Schneider cells Tfh cells total internal reflection microcopy fluorescence resonance energy transfer flow cytometry confocal microscopy two-photon microscopy antibodies

Editors and affiliations

  • Chaohong Liu
    • 1
  1. 1.Department of Pathogen BiologyHuazhong University of Science and TechnologyHuazhongChina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7473-3
  • Online ISBN 978-1-4939-7474-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site