Phage Display

Methods and Protocols

  • Michael Hust
  • Theam Soon Lim

Part of the Methods in Molecular Biology book series (MIMB, volume 1701)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Construction of Antibody Phage Display Libraries

    1. Front Matter
      Pages 1-1
    2. Jonas Kügler, Florian Tomszak, André Frenzel, Michael Hust
      Pages 3-24
    3. Noorsharmimi Omar, Theam Soon Lim
      Pages 25-44
    4. Johan Nilvebrant, Sachdev S. Sidhu
      Pages 45-60
    5. Arnaud Avril, Sebastian Miethe, Michael Hust, Thibaut Pelat
      Pages 83-112
    6. Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim
      Pages 133-146
    7. Julius Grzeschik, Doreen Könning, Steffen C. Hinz, Simon Krah, Christian Schröter, Martin Empting et al.
      Pages 147-167
    8. Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, Baptiste Monsion
      Pages 169-187
    9. Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt
      Pages 189-203
    10. Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, Eva Bosse-Doenecke
      Pages 205-238
    11. Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau
      Pages 239-253
    12. Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung
      Pages 255-269
  3. Selection Strategies for Antibodies

    1. Front Matter
      Pages 271-271
    2. Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel et al.
      Pages 273-284
    3. Angela Chiew Wen Ch’ng, Nurul Hamizah Binti Hamidon, Zoltán Konthur, Theam Soon Lim
      Pages 301-319
    4. Wieland Fahr, André Frenzel
      Pages 321-330
    5. Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora et al.
      Pages 365-380
    6. Andre ten Haaf, Stefan Gattenlöhner, Mehmet Kemal Tur
      Pages 381-391
    7. Tobias Unkauf, Michael Hust, André Frenzel
      Pages 393-407
  4. Complementary Approaches for Antibody Phage Display Selections

    1. Front Matter
      Pages 409-409
    2. Chao-Guang Chen, Georgina Sansome, Michael J. Wilson, Con Panousis
      Pages 447-461
    3. Anna Vaisman-Mentesh, Yariv Wine
      Pages 463-473
  5. Phage Display for Epitope Mapping and Identification of Biomarkers

    1. Front Matter
      Pages 475-475
    2. Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, Michael Hust
      Pages 477-495
    3. Gustavo Marçal Schmidt Garcia Moreira, Viola Fühner, Michael Hust
      Pages 497-518
    4. Milica Ciric, Filomena Ng, Jasna Rakonjac, Dragana Gagic
      Pages 519-534
  6. Back Matter
    Pages 561-562

About this book


This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field
of antibody phage display. This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.


scFv libraries macaque immune libraries IgNAR DARPINs array antibodies

Editors and affiliations

  • Michael Hust
    • 1
  • Theam Soon Lim
    • 2
  1. 1.Technische Universität BraunschweigBraunschweigGermany
  2. 2.Institute for Research in Molecular MedicineUniversiti Sains MalaysiaMinden, PenangMalaysia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7446-7
  • Online ISBN 978-1-4939-7447-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site