Methods and Protocols

  • Alice Pébay
  • Kursad Turksen

Part of the Methods in Molecular Biology book series (MIMB, volume 1697)

Table of contents

  1. Front Matter
    Pages i-x
  2. Melissa R. Pitman, Lorena T. Davies, Stuart M. Pitson
    Pages 9-20
  3. Alena P. Chumanevich, Piper A. Wedman, Carole A. Oskeritzian
    Pages 21-30
  4. Melina G. Castro, Ludmila E. Campos, Yamila I. Rodriguez, Sergio E. Alvarez
    Pages 117-131
  5. Phillip Callihan, Mohammed Alqinyah, Shelley B. Hooks
    Pages 141-151
  6. Guanghu Wang, Stefka D. Spassieva, Erhard Bieberich
    Pages 153-171
  7. Shrestha Priyadarsini, Sarah E. Nicholas, Dimitrios Karamichos
    Pages 173-180
  8. Back Matter
    Pages 181-182

About this book


This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.


Immunohistochemical Physiopathology Sphingolipids S1P Bioactive lysophospholipid Stem cells

Editors and affiliations

  • Alice Pébay
    • 1
  • Kursad Turksen
    • 2
  1. 1.Centre for Eye Research AustraliaThe University of MelbourneEast MelbourneAustralia
  2. 2.Ottawa Hospital Research InstituteOttawaCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7412-2
  • Online ISBN 978-1-4939-7413-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site