© 2018

High Content Screening

A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

  • Paul A. Johnston
  • Oscar J. Trask

Part of the Methods in Molecular Biology book series (MIMB, volume 1683)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Reagents and Kits for Measuring Cells and their Phenotypes

    1. Front Matter
      Pages 1-1
    2. Kevin M. Chambers, Bhaskar S. Mandavilli, Nick J. Dolman, Michael S. Janes
      Pages 21-31
    3. Bhaskar S. Mandavilli, Robert J. Aggeler, Kevin M. Chambers
      Pages 33-46
    4. Bhaskar S. Mandavilli, Michelle Yan, Scott Clarke
      Pages 47-57
    5. Nick J. Dolman, Brent A. Samson, Kevin M. Chambers, Michael S. Janes, Bhaskar S. Mandavilli
      Pages 59-71
  3. Selecting Microtiter Plates, Identify Imaging Artifacts, RNAi Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS

    1. Front Matter
      Pages 73-73
    2. Milan Esner, Felix Meyenhofer, Marc Bickle
      Pages 149-164
    3. Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O’Connell, Martyn Banks, Andrea Weston
      Pages 165-191
  4. Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis

    1. Front Matter
      Pages 193-193
    2. Verena Fetz, Roland H. Stauber, Shirley K. Knauer
      Pages 195-210
    3. Paul A. Johnston, Malabika Sen, Yun Hua, Daniel P. Camarco, Tong Ying Shun, John S. Lazo et al.
      Pages 229-244
    4. David Novo, Kaya Ghosh, Sean Burke
      Pages 245-266

About this book


This second edition details some of the recent trends in HCS/HCA/HCI.  New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field.


measure cells cell phenotypes HCS data data handling HCS/HCA/HCI

Editors and affiliations

  • Paul A. Johnston
    • 1
  • Oscar J. Trask
    • 2
  1. 1.Department of Pharmaceutical Sciences, School of PharmacyUniversity of PittsburghPittsburghUSA
  2. 2.Bahama Bio, LLCBahamaUSA

Bibliographic information

  • Book Title High Content Screening
  • Book Subtitle A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery
  • Editors Paul A. Johnston
    Oscar J. Trask
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-7355-2
  • Softcover ISBN 978-1-4939-8461-9
  • eBook ISBN 978-1-4939-7357-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XIII, 397
  • Number of Illustrations 11 b/w illustrations, 71 illustrations in colour
  • Topics Biotechnology
  • Buy this book on publisher's site