Argonaute Proteins

Methods and Protocols

  • Katsutomo Okamura
  • Kotaro Nakanishi

Part of the Methods in Molecular Biology book series (MIMB, volume 1680)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Pooja Gangras, Daniel M. Dayeh, Justin W. Mabin, Kotaro Nakanishi, Guramrit Singh
    Pages 1-28
  3. Megumi Shigematsu, Shozo Honda, Yohei Kirino
    Pages 65-73
  4. Panagiotis Alexiou, Manolis Maragkakis, Zissimos Mourelatos, Anastassios Vourekas
    Pages 87-100
  5. Shintaro Iwasaki, Yukihide Tomari
    Pages 131-143
  6. Hiroshi M. Sasaki, Hisashi Tadakuma, Yukihide Tomari
    Pages 145-164
  7. Kensaku Murano, Yuka W. Iwasaki, Haruhiko Siomi
    Pages 165-177
  8. Dmitry A. Kretov, Andrew M. Shafik, Daniel Cifuentes
    Pages 179-190
  9. Cai Zhang, Joonbae Seo, Takahisa Nakamura
    Pages 205-215
  10. Phillipe Loher, Aristeidis G. Telonis, Isidore Rigoutsos
    Pages 237-255
  11. Back Matter
    Pages 257-260

About this book


This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.


prokaryotes eukaryotes nucleic acids chaperon machinery Proteomics

Editors and affiliations

  • Katsutomo Okamura
    • 1
  • Kotaro Nakanishi
    • 2
  1. 1.Temasek Lifesciences LaboratoryNational University of SingaporeSingaporeSingapore
  2. 2.Department of Chemistry and BiochemistryThe Ohio State UniversityColumbusUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7338-5
  • Online ISBN 978-1-4939-7339-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site