Recombinant Glycoprotein Production

Methods and Protocols

  • Virgínia Picanço-Castro
  • Kamilla Swiech

Part of the Methods in Molecular Biology book series (MIMB, volume 1674)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Amanda Mizukami, Angelo Luis Caron, Virgínia Picanço-Castro, Kamilla Swiech
    Pages 1-14
  3. Upstream Processes

    1. Front Matter
      Pages 35-35
    2. Adam Nylen, Ming-Tang Chen
      Pages 37-48
    3. Aline de Sousa Bomfim, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Elisa Maria de Sousa Russo
      Pages 49-61
    4. Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro
      Pages 63-74
    5. Daniella C. Ventini-Monteiro, Renato M. Astray, Carlos A. Pereira
      Pages 87-94
    6. Challise J. Sullivan, Erik D. Pendleton, John Dresios
      Pages 95-107
    7. Tanja Holland, Johannes Felix Buyel
      Pages 129-146
    8. Yuzhou Fan, Daniel Ley, Mikael Rørdam Andersen
      Pages 147-161
    9. Agustina Gugliotta, Natalia Ceaglio, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero
      Pages 163-181
    10. Carlos Alexandre Breyer, Marcos Antonio de Oliveira, Adalberto Pessoa Jr
      Pages 183-191
  4. Downstream Processes

    1. Front Matter
      Pages 193-193
    2. Vladimir Granovski, Mario Soares Abreu-Neto, Dimas Tadeu Covas
      Pages 195-202
    3. Bruna Samham Archangelo, Elisa Maria de Sousa Russo
      Pages 203-209
    4. Vladimir Granovski, Marcela C. C. Freitas, Mario Soares Abreu-Neto, Dimas T. Covas
      Pages 221-226
  5. Formulation and Analytics

    1. Front Matter
      Pages 227-227
    2. Raquel Petrilli, Josimar O. Eloy, Robert J. Lee, Renata F. V. Lopez
      Pages 229-237
    3. Flávia Sousa, Pedro Fonte, Andreia Cruz, Patrick J. Kennedy, Inês Mendes Pinto, Bruno Sarmento
      Pages 239-253
    4. Pedro Fonte, Flávia Sousa, Bruno Sarmento
      Pages 255-274
    5. Edson Galdino do Nascimento Filho, Guilherme Pauperio Lanfredi, Mário Soares Abreu-Neto, Dimas Tadeu Covas, Vitor Marcel Faça
      Pages 275-282
  6. Back Matter
    Pages 283-285

About this book


This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field


Glycoengineering Glycosylation viral-particles gene therapy bifunctional antibodies

Editors and affiliations

  • Virgínia Picanço-Castro
    • 1
  • Kamilla Swiech
    • 2
  1. 1.Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  2. 2.School of Pharmaceutical Sciences of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7311-8
  • Online ISBN 978-1-4939-7312-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site