Single Molecule Analysis

Methods and Protocols

  • Erwin J. G. Peterman

Part of the Methods in Molecular Biology book series (MIMB, volume 1665)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Optical Tweezers

    1. Front Matter
      Pages 1-1
    2. Joost van Mameren, Gijs J. L. Wuite, Iddo Heller
      Pages 3-23
    3. Laurent Geffroy, Pierre Mangeol, Thierry Bizebard, Ulrich Bockelmann
      Pages 25-41
    4. Fatemeh Moayed, Roeland J. van Wijk, David P. Minde, Sander J. Tans
      Pages 43-51
    5. Lu Rao, Maren Hülsemann, Arne Gennerich
      Pages 53-89
  3. Single-Molecule Fluorescence Microscopy

    1. Front Matter
      Pages 91-91
    2. Siet M. J. L. van den Wildenberg, Bram Prevo, Erwin J. G. Peterman
      Pages 93-113
    3. Mauro Modesti
      Pages 115-134
    4. Aravindan Varadarajan, Felix Oswald, Yves J. M. Bollen
      Pages 135-144
    5. Jaap van Krugten, Erwin J. G. Peterman
      Pages 145-154
    6. Roderick P. Tas, Trusanne G. A. A. Bos, Lukas C. Kapitein
      Pages 155-171
    7. Fredrik Westerlund, Fredrik Persson, Joachim Fritzsche, Jason P. Beech, Jonas O. Tegenfeldt
      Pages 173-198
    8. Kathy R. Chaurasiya, Remus T. Dame
      Pages 217-239
  4. Atomic Force Microscopy and Other Force Methods

    1. Front Matter
      Pages 241-241
    2. Melissa C. Piontek, Wouter H. Roos
      Pages 243-258
    3. Małgorzata Grosbart, Dejan Ristić, Humberto Sánchez, Claire Wyman
      Pages 259-280
    4. Natalia Martín-González, Alvaro Ortega-Esteban, F. Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Pedro J. de Pablo
      Pages 281-296
    5. Douwe Kamsma, Gijs J. L. Wuite
      Pages 341-351
  5. Back Matter
    Pages 367-369

About this book


The aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule. 


Optical tweezers Single-Molecule Fluorescence (SMF) Atomic Force Microscopy Biomolecules Single-molecule biology

Editors and affiliations

  • Erwin J. G. Peterman
    • 1
  1. 1.Department of Physics & AstronomyVrije UniversiteitAmsterdamThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7270-8
  • Online ISBN 978-1-4939-7271-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site