Advertisement

© 2017

Super-Resolution Microscopy

Methods and Protocols

  • Holger Erfle
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 1663)

Table of contents

  1. Front Matter
    Pages i-xi
    PDF
  2. Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics
    Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle et al.
    Pages 1-13
  3. Managing the Introduction of Super-Resolution Microscopy into a Core Facility
    Jeffrey A. Kamykowski, Brian Storrie
    Pages 15-19
  4. Live-Cell STED Imaging with the HyPer2 Biosensor
    Natalia M. Mishina, Vsevolod V. Belousov
    Pages 21-28
  5. Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
    André Klauss, Carsten Hille
    Pages 29-44
  6. Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
    Mirelle J. T. ter Veer, Thomas Pfeiffer, U. Valentin Nägerl
    Pages 45-64
  7. STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
    Roman S. Erdmann, Derek Toomre, Alanna Schepartz
    Pages 65-78
    PDF
  8. Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
    Ulrike Engel
    Pages 79-94
  9. Correlative SIM-STORM Microscopy
    O. Burri, T. Laroche, R. Guiet, A. Seitz
    Pages 95-103
    PDF
  10. Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
    Patricia Bondia, Santiago Casado, Cristina Flors
    Pages 105-113
  11. Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
    Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann
    Pages 115-126
  12. Two-Color Single-Molecule Tracking in Live Cells
    Siegfried Hänselmann, Dirk-Peter Herten
    Pages 127-138
    PDF
  13. Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy
    Jan Philipp Eberle, Walter Muranyi, Holger Erfle, Manuel Gunkel
    Pages 139-152
  14. Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
    Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, Erik M. Jorgensen
    Pages 153-162
  15. Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
    Errin Johnson, Rainer Kaufmann
    Pages 163-177
  16. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
    Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothée Lionnet, Luke D. Lavis
    Pages 179-188
  17. Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step
    Susanna Maria Früh, Ingmar Schoen
    Pages 189-203
  18. Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
    Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman
    Pages 205-217
  19. Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
    Kem A. Sochacki, Justin W. Taraska
    Pages 219-230
  20. In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
    Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang et al.
    Pages 231-252
  21. Super-Resolution High Content Screening and Analysis
    K. Soliman
    Pages 253-259
  22. Back Matter
    Pages 261-262
    PDF

About this book

Introduction

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Keywords

Stimulated Emission Depletion Ground State Depletion Photo Activated Localization fluorescence STORM

Editors and affiliations

  • Holger Erfle
    • 1
  1. 1.High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening FacilityBioQuant, Heidelberg UniversityHeidelbergGermany

Bibliographic information

  • Book Title Super-Resolution Microscopy
  • Book Subtitle Methods and Protocols
  • Editors Holger Erfle
  • Series Title Methods in Molecular Biology
  • DOI https://doi.org/10.1007/978-1-4939-7265-4
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-7264-7
  • Softcover ISBN 978-1-4939-8435-0
  • eBook ISBN 978-1-4939-7265-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 262
  • Number of Illustrations 11 b/w illustrations, 37 illustrations in colour
  • Topics Imaging / Radiology
  • Buy this book on publisher's site

Reviews

“The aim is to provide a current overview of the advanced technologies and methods in super‐resolution microscopy. The book is written for novices and experienced investigators. It will be of much help for graduate students and postdoctoral students interested in super-resolution microscopy. The author is a credible authority in the field of super-resolution microscopy. … This book covers advanced, cutting-edge techniques related to super-resolution microscopy. The intended audience will find it of immense help.” (Omer Iqbal, Doody's Book Reviews, February, 2018)